基于液滴的光寻址蛋白质检测法。

Optically addressed droplet-based protein assay.

作者信息

Kotz Kenneth T, Gu Yu, Faris Gregory W

机构信息

Molecular Physics Laboratory, SRI International, 333 Ravenswood Avenue, Menlo Park, California 94025, USA.

出版信息

J Am Chem Soc. 2005 Apr 27;127(16):5736-7. doi: 10.1021/ja050249m.

DOI:10.1021/ja050249m

PMID:15839641

Abstract

We demonstrate a new method for performing protein assays with very small volumes ( approximately 1.7 muL to 14 pL). Using a laser to modify local surface energy, we manipulate, fuse, and mix droplets containing horseradish peroxidase and its substrates. A detection limit of approximately 30 attomoles of reacting enzyme was measured by optical absorption. We discuss the possibility of extending this lower limit to zeptomoles of enzyme.

摘要

我们展示了一种用于进行超微量蛋白质测定的新方法（体积约为1.7微升至14皮升）。利用激光改变局部表面能，我们操控、融合并混合含有辣根过氧化物酶及其底物的液滴。通过光吸收测量得到反应酶的检测限约为30阿托摩尔。我们讨论了将此下限扩展至zeptomoles酶的可能性。