Theodore D R, Meier-Ruge W, Abraham J
Department of Neurological Sciences, Christian Medical College and Hospital, Vellore, India.
Microvasc Res. 1992 Mar;43(2):147-55. doi: 10.1016/0026-2862(92)90013-f.
Focal cerebral ischemia was produced in monkeys by transorbital occlusion of the right middle cerebral artery. Following this, in one group of animals the total microvasculature, including both perfused and nonperfused vessels of the opposite caudate nucleus and insula, was examined by alkaline phosphatase staining of the endothelium. In another group, the patency of the microvascular bed was visualized by india ink perfusion. The number, diameter, and length of visualized vessels were measured by means of a Wild ASBA image analysis system. The perfused patient microvascular bed was significantly reduced in both insula and caudate nucleus in the supposedly normal left side, although the total microvascular volume showed an increase at 4 and 12 hr in the insula and at 48 hr in the caudate nucleus. Reduced perfusion in the hemisphere opposite to the occluded middle cerebral artery provides an anatomical substrate for the phenomenon of "diaschisis."
通过经眶闭塞右侧大脑中动脉在猴子身上制造局灶性脑缺血。在此之后，一组动物通过内皮碱性磷酸酶染色检查了对侧尾状核和岛叶的整个微血管系统，包括灌注和未灌注的血管。另一组通过印度墨汁灌注观察微血管床的通畅情况。使用Wild ASBA图像分析系统测量可见血管的数量、直径和长度。在假定正常的左侧，岛叶和尾状核的灌注微血管床均显著减少，尽管在岛叶4小时和12小时以及尾状核48小时时总微血管体积有所增加。闭塞大脑中动脉对侧半球的灌注减少为“交叉性小脑失联络”现象提供了解剖学基础。
