Ishikawa M, Handa H, Kim S, Hirai O, Yoshida S
Neurol Res. 1984 Dec;6(4):155-8. doi: 10.1080/01616412.1984.11739681.
In order to reveal the correlation of energy failure and neurotransmitter metabolism in the acute stage of incomplete cerebral ischaemia, the change of NADH (reduced form of nicotinamide adenine dinucleotide) and brain dopamine was studied histochemically using the same specimen on the unilateral carotid ligation of mongolian gerbils. The severity of clinical symptoms was well correlated with the extent and intensity of NADH fluorescence. Dopamine in the caudate nucleus on the ligated side decreased heterogeneously shortly after the cerebral ischaemia. Preservation of dopamine was noted around the small vessels with thick walls. Thus, heterogeneity of dopamine decrease appears to be closely related to the structure of microvasculature. It was seen in areas of heterogeneously increased NADH, but also in areas of its homogeneous increase within 5 to 60 min after carotid ligation. This microheterogeneity of brain dopamine may be due to the difference of perfusion pressure at the microcirculation in the acute stage of incomplete cerebral ischaemia.
为了揭示不完全性脑缺血急性期能量衰竭与神经递质代谢的相关性，在蒙古沙土鼠单侧颈动脉结扎后，使用同一标本对还原型烟酰胺腺嘌呤二核苷酸（NADH）和脑多巴胺的变化进行了组织化学研究。临床症状的严重程度与NADH荧光的范围和强度密切相关。脑缺血后不久，结扎侧尾状核中的多巴胺呈异质性减少。在壁厚的小血管周围观察到多巴胺的保留。因此，多巴胺减少的异质性似乎与微血管结构密切相关。在颈动脉结扎后5至60分钟内，在NADH异质性增加的区域以及其均匀增加的区域均可见到这种情况。脑多巴胺的这种微观异质性可能是由于不完全性脑缺血急性期微循环灌注压力的差异所致。
