Ito Takuya, Motohashi Reiko, Kuromori Takashi, Noutoshi Yoshiteru, Seki Motoaki, Kamiya Asako, Mizukado Saho, Sakurai Tetsuya, Shinozaki Kazuo
Plant Molecular Biology Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, 305-0074 Japan.
Plant Cell Physiol. 2005 Jul;46(7):1149-53. doi: 10.1093/pcp/pci112. Epub 2005 Apr 19.
We report here the generation of an additional collection of Dissociation (Ds) transposon-tagged, sequence-indexed lines of Arabidopsis thaliana. Our RIKEN Ds insertion collection now totals 17,668 lines. Our collection has preferential insertions in chromosomes 1 and 5, because Ds was transposed from start loci on those chromosomes (11,854 and 5,814 lines, respectively). We describe here features of the latter 5,814 lines. The former 11,854 lines have been described previously. We have created a searchable database of the insertion sites and mutated genes (http://rarge.gsc.riken.jp/), and are depositing these lines in the RIKEN BioResource Center (http://www.brc.riken.go.jp/lab/epd/Eng/). Our collection of these mutants will contribute to progress in functional genomics of plants.
我们在此报告拟南芥解离（Ds）转座子标签化、序列索引系的新增集合的构建情况。我们的理化学研究所Ds插入系集合目前总计17668个株系。我们的集合在第1和第5号染色体上有优先插入，因为Ds是从这些染色体上的起始位点转座而来的（分别为11854和5814个株系）。我们在此描述后5814个株系的特征。前11854个株系此前已作过描述。我们创建了一个可搜索的插入位点和突变基因数据库（http://rarge.gsc.riken.jp/），并将这些株系保藏于理化学研究所生物资源中心（http://www.brc.riken.go.jp/lab/epd/Eng/）。我们对这些突变体的集合将有助于植物功能基因组学的进展。
