通过体外DNA扩增检测和鉴别肺炎支原体和生殖支原体。
Detection and discrimination of Mycoplasma pneumoniae and Mycoplasma genitalium by the in vitro DNA amplification.
作者信息
Sasaki Y, Shintani M, Shimada T, Watanabe H, Sasaki T
机构信息
Department of General Biologics Control, National Institute of Health, Tokyo, Japan.
出版信息
Microbiol Immunol. 1992;36(1):21-7. doi: 10.1111/j.1348-0421.1992.tb01638.x.
By using the primers designed on the bases of the sequences of the 16S rRNA genes of Mycoplasma pneumoniae and Mycoplasma genitalium, respectively, specific and sensitive in vitro DNA amplification assay system for the detection and discrimination of these two mycoplasmas was established. The detection limit of the assay was 100 cells for M. pneumoniae and 1,000 cells for M. genitalium. Neither other human mycoplasmas nor oral bacteria existing in human saliva showed any cross-reactions with these primers.
分别根据肺炎支原体和生殖支原体16S rRNA基因序列设计引物，建立了用于检测和区分这两种支原体的特异性强且灵敏的体外DNA扩增检测系统。该检测方法对肺炎支原体的检测限为100个细胞，对生殖支原体的检测限为1000个细胞。其他人型支原体以及人唾液中存在的口腔细菌与这些引物均未出现任何交叉反应。
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