Uchida T, Suzuki K, Hayashi N, Iida F, Hara T, Oo S S, Wang C K, Shikata T, Ichikawa M, Rikihisa T
Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Microbiol Immunol. 1992;36(1):67-79. doi: 10.1111/j.1348-0421.1992.tb01643.x.
Viral hepatitis E is endemic, frequently provoking epidemic outbreaks in many developing countries. We have attempted to clone the viral genome and to develop an antibody assay system. A lambda gt11 cDNA library was constructed from the bile juice containing putative causative viruses and was immunoscreened by the antisera obtained from patients and monkeys infected with hepatitis E. Three virus-specific clones were isolated and were revealed to overlap one another in sequence, with 1,459 nucleotides in total length. These clones direct the synthesis of polypeptides probably having common immunological epitope(s). Immunoplaque assay revealed the occurrence of antibodies against this epitope in the sera from experimental monkeys with the convalescent phase and from patients of Myanmar, Nepal and India. The data indicate that the cDNA fragments are useful for immunodiagnosis of hepatitis E.
戊型病毒性肝炎呈地方性流行，在许多发展中国家常常引发疫情爆发。我们试图克隆病毒基因组并开发一种抗体检测系统。从含有假定致病病毒的胆汁中构建了一个λgt11 cDNA文库，并用感染戊型肝炎的患者和猴子的抗血清进行免疫筛选。分离出三个病毒特异性克隆，发现它们在序列上相互重叠，全长1459个核苷酸。这些克隆指导合成可能具有共同免疫表位的多肽。免疫斑试验显示，处于恢复期的实验猴以及缅甸、尼泊尔和印度患者的血清中存在针对该表位的抗体。数据表明，这些cDNA片段可用于戊型肝炎的免疫诊断。
