Ichikawa M, Araki M, Rikihisa T, Uchida T, Shikata T, Mizuno K
Chemo-Sero Therapeutic Research Institute, Kikuchi Laboratories, Kumamoto.
Microbiol Immunol. 1991;35(7):535-43. doi: 10.1111/j.1348-0421.1991.tb01584.x.
The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with beta-galactosidase (beta-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.
