Fu Eugene S, Saporta Samuel
Department of Anesthesiology, University of Miami School of Medicine, Miami, Florida 33136, USA.
J Neurosurg Anesthesiol. 2005 Apr;17(2):82-5. doi: 10.1097/01.ana.0000163199.10365.38.
Interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) are major inflammatory cytokines produced after spinal cord injury (SCI). This study sought to evaluate the effects of methylprednisolone (MP) on IL-1beta and IL-6 protein in spinal cord tissue following SCI. Halothane-anesthetized, female Sprague-Dawley rats weighing (280-320 g) underwent laminectomy at T7-T8. No lesions were produced in animals in the saline control and MP control groups. SCI was induced by temporary placement of an aneurysm clip at T7-T8, with a closing pressure of 55 g at the spinal level of T7-T8, resulting in spinal cord compression for one minute. Animals with SCI were treated with MP (30 mg/kg sc) or an equal volume of saline. IL-1beta and IL-6 spinal cord protein were measured by enzyme-linked immunosorbent assays (ELISA). Data were summarized as mean +/- SD and compared by two-way analysis of variance (ANOVA). IL-1beta and IL-6 levels were elevated in the SCI + Saline animals (P < 0.01) compared with saline control, MP control, and SCI + MP-treated animals. The rise in IL-1beta and IL-6 levels after SCI was blunted after administration of MP, suggesting an interaction between glucocorticosteroids and the cytokine cascade after spinal cord trauma. Further evaluation of the effects of MP on the cytokine cascade may be important in assessing whether or not the anti-inflammatory effects of glucocorticosteroids confer neuroprotection after SCI.
白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）是脊髓损伤（SCI）后产生的主要炎性细胞因子。本研究旨在评估甲基泼尼松龙（MP）对SCI后脊髓组织中IL-1β和IL-6蛋白的影响。将体重（280 - 320 g）的氟烷麻醉雌性Sprague-Dawley大鼠在T7 - T8节段进行椎板切除术。生理盐水对照组和MP对照组动物未产生损伤。通过在T7 - T8节段临时放置动脉瘤夹诱导SCI，在T7 - T8脊髓水平施加55 g的闭合压力，导致脊髓压迫1分钟。SCI动物用MP（30 mg/kg皮下注射）或等体积生理盐水治疗。通过酶联免疫吸附测定（ELISA）测量脊髓中IL-1β和IL-6蛋白。数据总结为平均值±标准差，并通过双向方差分析（ANOVA）进行比较。与生理盐水对照组、MP对照组和SCI + MP治疗组动物相比，SCI +生理盐水组动物的IL-1β和IL-6水平升高（P < 0.01）。给予MP后，SCI后IL-1β和IL-6水平的升高受到抑制，提示糖皮质激素与脊髓损伤后细胞因子级联反应之间存在相互作用。进一步评估MP对细胞因子级联反应的影响对于评估糖皮质激素的抗炎作用在SCI后是否赋予神经保护可能很重要。
