Abdanipour Alireza, Tiraihi Taki, Taheri Taher, Kazemi Hadi
Dept. of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Iran Biomed J. 2013;17(4):214-20. doi: 10.6091/ibj.1213.2013.
The present study was designed to evaluate the secondary microglial activation processes after spinal cord injury (SCI).
A quantitative histological study was performed to determine ED-1 positive cells, glial cell density, and cavitation size in untreated SCI rats at days 1, 2, and 4, and weeks 1, 2, 3, and 4.
The results of glial cell quantification along the 4900-µm long injured spinal cord showed a significant increase in glial cell density percentage at day 2 as compared to other days. Whereas the highest increase in ED-1 immunoreactive cells (monocyte/phagocyte marker in rats) was observed at day 2 (23.15%) post-injury. Evaluation of cavity percentage showed a significant difference between weeks 3 and 4 post-injury groups.
This study provides a new insight into the multiphase immune response to SCI, including cellular inflammation, macrophages/microglia activation, glial cell density, and cavitation. Better understanding of the inflammatory processes associated with acute SCI would permit the development of better therapeutic strategies.
本研究旨在评估脊髓损伤（SCI）后的继发性小胶质细胞激活过程。
进行了一项定量组织学研究，以确定未治疗的SCI大鼠在第1、2和4天以及第1、2、3和4周时ED-1阳性细胞、胶质细胞密度和空洞大小。
沿4900μm长的损伤脊髓进行的胶质细胞定量结果显示，与其他天数相比，第2天的胶质细胞密度百分比显著增加。而损伤后第2天观察到ED-1免疫反应性细胞（大鼠单核细胞/吞噬细胞标志物）增加最多（23.15%）。空洞百分比评估显示损伤后第3周和第4周组之间存在显著差异。
本研究为SCI的多相免疫反应提供了新的见解，包括细胞炎症、巨噬细胞/小胶质细胞激活、胶质细胞密度和空洞形成。更好地理解与急性SCI相关的炎症过程将有助于开发更好的治疗策略。
