Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Neuroscience Research Center (NRC), Iran University of Medical Sciences, Tehran, Iran; Department of Medical Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Neuropeptides. 2018 Aug;70:16-25. doi: 10.1016/j.npep.2018.05.002. Epub 2018 May 7.
Spinal cord injury (SCI) is a serious and complex medical condition that can happen to anyone. At present, therapy mainly focuses on rehabilitation and pharmacological treatment, such as methylprednisolone (MP). Supra-spinal changes in certain structures, such as the cerebellum, that receive many afferents from the spinal cord might be one reason for unsuccessful therapeutic outcomes. Recently, the expression of FNDC5 was reported in cerebellar Purkinje cells as a possible neuroprotective agent. In the present study, we considered the expression of FNDC5 in Purkinje cells following SCI with and without MP administration in adult rats with SCI. Thirty-five adult male rats were used in this study. The animals were randomly allocated into five groups, including SCI, spinal cord injury with methylprednisolone treatment (SCI + MP), operation sham, control, and operation sham with MP. Induction of SCI was achieved by using special clips to compress the spinal cord at a determined level. After a certain interval time, the animals underwent study for FNDC5 expression, apoptosis by using immunohistochemistry, Western blotting, and TUNEL and Nissl staining. Our results showed a significant decrease in the number of Purkinje cells following SCI. Therapy with MP inhibits apoptosis in irFNDC5 Purkinje cells and restores them. Expression of FNDC5 significantly increased in SCI and decreased following MP therapy. We also showed other cerebellar cells with FNDC5 immunoreactivity in the two other cerebellar layers that were firstly reported. Since irisin is known as a plasma product of FNDC5, we think it might be a plasma marker following therapeutic efforts for SCI; however, it needs further research. In addition, it is possible that changes in FNDC5 expression in Purkinje cells might be related to neurogenesis in the cerebellum with unknown mechanisms.
脊髓损伤（SCI）是一种严重且复杂的医疗状况，可能发生在任何人身上。目前，治疗主要集中在康复和药物治疗上，例如甲基强的松龙（MP）。脊髓传入纤维较多的某些结构，如小脑，其上位变化可能是治疗效果不佳的原因之一。最近，FNDC5 在小脑浦肯野细胞中的表达被报道为一种可能的神经保护剂。在本研究中，我们考虑了成年 SCI 大鼠中 SCI 后有无 MP 给药时 FNDC5 在浦肯野细胞中的表达。本研究使用了 35 只成年雄性大鼠。将动物随机分为五组，包括 SCI 组、SCI 加 MP 治疗组、手术假手术组、对照组和手术假手术加 MP 组。通过使用特殊夹在特定水平压迫脊髓来诱导 SCI。在一定的间隔时间后，对动物进行 FNDC5 表达、免疫组织化学、Western blot、TUNEL 和尼氏染色检测凋亡。结果显示，SCI 后浦肯野细胞数量明显减少。MP 治疗抑制 irFNDC5 浦肯野细胞凋亡并使其恢复。FNDC5 在 SCI 中表达显著增加，而在 MP 治疗后表达减少。我们还在小脑的另外两层中首次报道了具有 FNDC5 免疫反应性的其他小脑细胞。由于鸢尾素被认为是 FNDC5 的血浆产物，我们认为它可能是 SCI 治疗后血浆的标志物；然而，这需要进一步的研究。此外，FNDC5 在浦肯野细胞中的表达变化可能与小脑的神经发生有关，其机制尚不清楚。
