Bielanski A
Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa/Nepean, Ont., Canada K2H 8P9.
Cryobiology. 2005 Apr;50(2):206-10. doi: 10.1016/j.cryobiol.2004.12.004.
Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos and semen in dewars designed for short-term storage and transportation of biological specimens. In this study transmission of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, BVDV, and BHV-1 was examined from: (1) contaminated dry shippers to germplasm; (2) between contaminated and non-contaminated cryopreserved germplasm; and (3) between stock culture of pathogenic agents and germplasm. Contaminated and non-contaminated samples of embryos and semen were stored in proximity in the vapour phase LN in open containers for 7 days prior to testing for the presence of microbes. The results showed that there was no cross-contamination from either the contaminated dewars to germplasm or between contaminated and non-contaminated samples of embryos and semen during 7 days storage under LN vapours. The outcome of our investigation indicates that VPLN is a safe means for short-term storage of embryos and semen in dry shipper dewars commonly used for transportation of bovine germplasm.
种质的冷冻保存是预防胚胎移植和人工授精过程中疾病传播的一个重要因素。在此，我们报告一项关于在用于生物样本短期储存和运输的杜瓦瓶中，液氮气相（VPLN）将特定细菌和病毒病原体传播至胚胎和精液的调查结果。在本研究中，检测了铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌、牛病毒性腹泻病毒（BVDV）和牛疱疹病毒1型（BHV-1）的传播情况，具体涉及：（1）从受污染的干式运输罐到种质；（2）在受污染和未受污染的冷冻保存种质之间；以及（3）在病原体的原代培养物和种质之间。在检测微生物存在之前，将受污染和未受污染的胚胎及精液样本在气相液氮中敞口容器内相邻存放7天。结果表明，在液氮蒸汽下储存7天期间，无论是受污染的杜瓦瓶到种质，还是胚胎和精液的受污染与未受污染样本之间，均未发生交叉污染。我们的调查结果表明，VPLN是在常用于运输牛种质的干式运输罐杜瓦瓶中短期储存胚胎和精液的一种安全方法。
