Lee Robert Kuo-Kuang, Ho Hsin-Yi, Yu San-Li, Lu Chung-Hao
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei 10449, Taiwan, Republic of China.
J Assist Reprod Genet. 2005 Feb;22(2):95-101. doi: 10.1007/s10815-005-1499-z.
To investigate follicle survival and developmental potential with IVF of cryopreserved, subcutaneously transplanted mouse ovarian tissue.
Fresh and frozen mouse ovarian tissue was autologously transplanted into subcutaneous tissue. Two weeks after the transplantation, the morphology and histology of the fresh and frozen grafts were compared. Superovulation and IVF was performed to evaluate the fertility potential of the frozen ovarian graft.
Both fresh and frozen grafts of ovarian tissue survived in 14 of 16 mice (88%). Morphologically, both types of grafts resembled fresh ovarian tissue and contained follicles at all stages of folliculogenesis. A total of 73% of follicles in fresh grafts and 62% in frozen grafts survived after transplantation compared with fresh ovarian tissue. Sixteen ICR mice underwent superovulation. A total of 56 oocytes from antral follicles were recovered from the subcutaneously transplanted cryopreserved ovarian tissue. Fourteen (25%) oocytes were in metaphase II stage, 6 were fertilized by IVF, and 2 progressed to the blastocyst stage.
Cryopreservation and subcutaneous transplantation of ovarian tissue provides a possible means of fertility preservation. The main loss of follicles occurred during grafting rather than during freezing and thawing.
研究经冷冻保存并皮下移植的小鼠卵巢组织进行体外受精时卵泡的存活情况及发育潜能。
将新鲜和冷冻的小鼠卵巢组织自体移植到皮下组织。移植两周后，比较新鲜和冷冻移植物的形态学和组织学。进行超排卵和体外受精以评估冷冻卵巢移植物的生育潜能。
16只小鼠中有14只（88%）的新鲜和冷冻卵巢移植物均存活。形态学上，两种类型的移植物均类似于新鲜卵巢组织，且含有卵泡发生各阶段的卵泡。与新鲜卵巢组织相比，移植后新鲜移植物中73%的卵泡和冷冻移植物中62%的卵泡存活。16只ICR小鼠进行了超排卵。从皮下移植的冷冻保存卵巢组织中总共回收了56个来自窦卵泡的卵母细胞。14个（25%）卵母细胞处于中期II期，6个经体外受精受精，2个发育至囊胚期。
卵巢组织的冷冻保存和皮下移植提供了一种可能的生育力保存方法。卵泡的主要损失发生在移植过程中，而非冻融过程中。
