Candy C J, Wood M J, Whittingham D G
Medical Research Council Experimental Embryology and Teratology Unit, St George's Hospital Medical School, London, UK.
Hum Reprod. 1995 Sep;10(9):2334-8. doi: 10.1093/oxfordjournals.humrep.a136295.
Pieces of marmoset ovary were frozen by slow cooling in 1.5 M dimethylsulphoxide. The follicles in fresh and frozen tissue were counted and examined for morphological appearance in stained serial sections. The proportion of normal follicles was similar in fresh tissue and frozen tissue examined immediately after thawing. Follicles at all stages of folliculogenesis up to the small antral stage survived freezing and thawing. Fresh and frozen tissue was transplanted underneath the kidney capsules of ovariectomized immunodeficient mice. The establishment of grafts was similar, and oestrogenic activity (cornification of the vaginal epithelium) was observed in the recipients 20 and 16 days after transplantation of fresh and frozen grafts respectively. The total number of follicles and the proportion of normal follicles were similar in fresh and frozen grafts. Grafts of frozen tissue recovered between 7 and 15 days after transfer contained follicles up to the small antral stage of development. Grafts recovered between 21 and 32 days contained follicles at all stages of folliculogenesis, including large antral follicles (1-2 mm diameter). Our results suggest that freezing and thawing do not substantially damage marmoset ovarian tissue, and the cryopreserved tissue retains its ability to support the development of large antral follicles.
将狨猴卵巢组织块置于1.5 M二甲基亚砜中通过缓慢冷却进行冷冻。对新鲜组织和冷冻组织中的卵泡进行计数，并在染色的连续切片中检查其形态外观。解冻后立即检查的新鲜组织和冷冻组织中正常卵泡的比例相似。卵泡发生各阶段直至小腔卵泡阶段的卵泡在冷冻和解冻后均存活。将新鲜组织和冷冻组织移植到卵巢切除的免疫缺陷小鼠的肾包膜下。移植情况相似，分别在新鲜移植物和冷冻移植物移植后20天和16天在受体中观察到雌激素活性（阴道上皮角质化）。新鲜移植物和冷冻移植物中的卵泡总数和正常卵泡比例相似。在转移后7至15天回收的冷冻组织移植物中含有发育至小腔卵泡阶段的卵泡。在21至32天回收的移植物中含有卵泡发生各阶段的卵泡，包括大腔卵泡（直径1 - 2毫米）。我们的结果表明，冷冻和解冻不会对狨猴卵巢组织造成实质性损害，并且冷冻保存的组织保留了支持大腔卵泡发育的能力。
