van Knippenberg Ingeborg, Goldbach Rob, Kormelink Richard
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709PD Wageningen, The Netherlands.
Virus Res. 2005 Jun;110(1-2):125-31. doi: 10.1016/j.virusres.2005.01.012.
The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3'-end of the intergenic hairpin, i.e. position 1568-1574 for N and position 1852-1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3'-end. The potential involvement of the 3'-end structure in transcription termination is discussed.
番茄斑萎病毒双义M和S RNA片段含有一个富含A/U的基因间隔区，预计可形成稳定的发夹结构。通过RT-PCR克隆和Northern印迹上的大小估计，在体外转录系统中合成的S片段编码的N和NSs mRNA的转录终止位点大致定位到基因间隔发夹的3'端，即N为1568 - 1574位，NSs为1852 - 1839位。这表明这些病毒转录本在其3'端含有一个预测的茎环结构。讨论了3'端结构在转录终止中的潜在作用。
