Rao Xueqin, Wu Zhuyan, Li Yuan
Laboratory of Plant Virology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
Virus Genes. 2013 Jun;46(3):576-80. doi: 10.1007/s11262-013-0885-3. Epub 2013 Mar 21.
The complete genome of a Watermelon silver mottle virus (WSMoV) (genus Tospovirus, family Bunyaviridae) isolate (WSMoV-GZ) from Guangdong province, China was sequenced. The genomes of WSMoV-GZ contained 3,603, 4,909, and 8,914 nt of small (S), medium (M), and large (L) RNA segments, respectively, and had a genomic organization characteristic of members of the genus Tospovirus. The amino acid sequence of the nucleocapsid (N) protein, S RNA-encoded nonstructural (NSs) protein, M RNA-encoded nonstructural (NSm) protein, Gn/Gc glycoprotein precursor, and RNA-dependent RNA polymerase (RdRp) protein showed 94.3-97.5 % identity with those of other WSMoV isolates. Phylogenetic analysis showed that the N protein of WSMoV-GZ was clustered together with those of the WSMoV isolates. The full sequence of WSMoV-GZ provides a reference genome for comparison with other tospoviruses.
对来自中国广东省的一株西瓜银斑驳病毒（WSMoV，番茄斑萎病毒属，布尼亚病毒科）分离株（WSMoV-GZ）的全基因组进行了测序。WSMoV-GZ的基因组分别包含3603、4909和8914个核苷酸的小（S）、中（M）和大（L）RNA片段，具有番茄斑萎病毒属成员的基因组结构特征。核衣壳（N）蛋白、S RNA编码的非结构（NSs）蛋白、M RNA编码的非结构（NSm）蛋白、Gn/Gc糖蛋白前体和RNA依赖的RNA聚合酶（RdRp）蛋白的氨基酸序列与其他WSMoV分离株的氨基酸序列具有94.3%-97.5%的同一性。系统发育分析表明，WSMoV-GZ的N蛋白与WSMoV分离株的N蛋白聚集在一起。WSMoV-GZ的全序列为与其他番茄斑萎病毒进行比较提供了参考基因组。
