Josefsson Elisabet, Juuti Katri, Bokarewa Maria, Kuusela Pentti
Department of Rheumatology, University of Göteborg, Guldhedsgatan 10, S-413 46 Göteborg, Sweden.
Infect Immun. 2005 May;73(5):2812-7. doi: 10.1128/IAI.73.5.2812-2817.2005.
Pls, a surface protein of certain methicillin-resistant Staphylococcus aureus strains, is associated with poor bacterial adherence to solid-phase fibronectin and immunoglobulin G, as well as with reduced invasion of cultured epithelial cells. Here the importance of Pls for the development of septic arthritis and sepsis was investigated by using a mouse model. Mice inoculated with a pls knockout mutant developed a much milder arthritis and showed less grave weight reduction than mice infected with the wild-type Pls(+) clinical isolate. Also, the pls mutant induced a significantly lower frequency of mortality than the wild-type strain. The bacterial load of the kidneys was larger in mice infected with the Pls(+) strain than in animals challenged with the pls mutant. However, there was no evident inflammatory effect due to the Pls molecule alone, as indicated by knee injection of purified Pls. In conclusion, the results show that Pls is a virulence factor for septic arthritis and sepsis.
某些耐甲氧西林金黄色葡萄球菌菌株的表面蛋白Pls，与细菌对固相纤连蛋白和免疫球蛋白G的黏附性差有关，也与培养的上皮细胞侵袭减少有关。在此，利用小鼠模型研究了Pls在脓毒性关节炎和脓毒症发生发展中的重要性。接种pls基因敲除突变体的小鼠发生的关节炎要轻得多，体重减轻也比感染野生型Pls(+)临床分离株的小鼠轻。此外，pls突变体诱导的死亡率显著低于野生型菌株。感染Pls(+)菌株的小鼠肾脏中的细菌载量比用pls突变体攻击的动物中的细菌载量大。然而，如通过膝关节注射纯化的Pls所示，单独的Pls分子没有明显的炎症效应。总之，结果表明Pls是脓毒性关节炎和脓毒症的一种毒力因子。
