Tringe Susannah Green, von Mering Christian, Kobayashi Arthur, Salamov Asaf A, Chen Kevin, Chang Hwai W, Podar Mircea, Short Jay M, Mathur Eric J, Detter John C, Bork Peer, Hugenholtz Philip, Rubin Edward M
Department of Energy (DOE) Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.
Science. 2005 Apr 22;308(5721):554-7. doi: 10.1126/science.1107851.
The species complexity of microbial communities and challenges in culturing representative isolates make it difficult to obtain assembled genomes. Here we characterize and compare the metabolic capabilities of terrestrial and marine microbial communities using largely unassembled sequence data obtained by shotgun sequencing DNA isolated from the various environments. Quantitative gene content analysis reveals habitat-specific fingerprints that reflect known characteristics of the sampled environments. The identification of environment-specific genes through a gene-centric comparative analysis presents new opportunities for interpreting and diagnosing environments.
微生物群落的物种复杂性以及培养代表性分离株面临的挑战使得获取组装好的基因组变得困难。在这里，我们使用通过对从各种环境中分离的DNA进行鸟枪法测序获得的大量未组装序列数据，来表征和比较陆地和海洋微生物群落的代谢能力。定量基因含量分析揭示了反映采样环境已知特征的特定栖息地指纹。通过以基因为中心的比较分析来鉴定环境特异性基因，为解释和诊断环境提供了新的机会。
