Li Hai-yan, Zhu Ling-xiang, Mao Ai-jun, Dong Zhi-yang
State Key Laboratory of Microbio Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wei Sheng Wu Xue Bao. 2005 Feb;45(1):135-8.
The cDNA sequence of beta-xylanase gene (xynB) was cloned from Aspergillus niger UV-11. It was inserted into the yeast expression vector and the recombinant plasmid pAX2 was obtained. The plasmid pAX2 was introduced into an industrial S. cerevisiae 2.346 and integrated into yeast genome by co-transformation of a YEPtype plasmid pBEJ16 carrying G418 resistance. The stable engineered yeast strain XY2 was obtained. It could express and secret extracellular xylanase, and enhance the alcohol production in wheat flour fermentation compared with the host strain S. cerevisiae 2.346.
从黑曲霉UV-11中克隆了β-木聚糖酶基因（xynB）的cDNA序列。将其插入酵母表达载体，获得重组质粒pAX2。将质粒pAX2导入工业酿酒酵母2.346，并通过携带G418抗性的YEP型质粒pBEJ16共转化整合到酵母基因组中。获得了稳定的工程酵母菌株XY2。它可以表达并分泌细胞外木聚糖酶，与宿主菌株酿酒酵母2.346相比，在小麦粉发酵中能提高酒精产量。
