Cid Joan, Ortín Xavier, Pinacho Asunción, Parra Rafael, Contreras Enric, Elies Enric
Transfusion Center and Tissue Bank, Barcelona, Spain.
Transfusion. 2005 May;45(5):694-7. doi: 10.1111/j.1537-2995.2005.04369.x.
Our reference adsorption procedure is to autoadsorb serum with ZZAP-pretreated patients' red cells (RBCs), although it is time-consuming. The aim of this study was to evaluate the use of polyethylene glycol (PEG) for performing autologous adsorption procedures without pretreatment of patients' RBCs.
Equal volumes of patient's plasma, patient's RBCs, and PEG were mixed. This mixture was incubated for 15 minutes at 37 degrees C, and the adsorbed plasma-PEG mixture was immediately harvested to proceed to the antiglobulin test with anti-immunoglobulin G. The ZZAP-adsorption procedure was performed in our reference laboratory.
Thirty-one samples were detected with warm autoantibodies in pretransfusion testing. A total number of 58 autologous adsorption procedures were performed with PEG in 870 minutes (mean, 28 min) and alloantibodies were detected in 4 (13%) cases (anti-E in 2 cases and anti-E + K in 2 cases). Our reference laboratory performed a total number of 61 adsorption procedures with ZZAP in 3660 minutes (mean, 118 min) and detected the same alloantibodies specificities.
The PEG autologous adsorption procedure is an efficient method of enhancing autoantibody adsorption and alloantibody detection and decreasing the labor-intensive testing required by the presence of serum warm autoantibodies in pretransfusion samples.
我们的参考吸附程序是用ZZAP预处理患者的红细胞（RBC）对血清进行自身吸附，尽管该方法耗时较长。本研究的目的是评估使用聚乙二醇（PEG）在不预处理患者红细胞的情况下进行自身吸附程序的效果。
将等量的患者血浆、患者红细胞和PEG混合。该混合物在37℃下孵育15分钟，然后立即收集吸附后的血浆-PEG混合物，用抗免疫球蛋白G进行抗球蛋白试验。ZZAP吸附程序在我们的参考实验室进行。
在输血前检测中，有31份样本检测出温自身抗体。共使用PEG进行了58次自身吸附程序，耗时870分钟（平均28分钟），4例（13%）检测出同种抗体（2例抗-E，2例抗-E + K）。我们的参考实验室使用ZZAP共进行了61次吸附程序，耗时3660分钟（平均118分钟），检测出相同的同种抗体特异性。
PEG自身吸附程序是一种有效的方法，可增强自身抗体吸附和同种抗体检测，并减少输血前样本中血清温自身抗体导致的劳动强度大的检测工作。
