壳聚糖纳米粒作为甘草酸铵的新型给药系统。

Chitosan nanoparticles as a novel delivery system for ammonium glycyrrhizinate.

作者信息

Wu Yan, Yang Wuli, Wang Changchun, Hu Jianhua, Fu Shoukuan

机构信息

Key Laboratory of Molecular Engineering of Polymers of Educational Ministry, Department of Macromolecular Science, Fudan University, Shanghai 200433, People's Republic of China.

出版信息

Int J Pharm. 2005 May 13;295(1-2):235-45. doi: 10.1016/j.ijpharm.2005.01.042.

DOI:10.1016/j.ijpharm.2005.01.042

PMID:15848008

Abstract

The ammonium glycyrrhizinate-loaded chitosan nanoparticles were prepared by ionic gelation of chitosan with tripolyphosphate anions (TPP). The particle size and zeta potential of nanoparticles were determined, respectively, by dynamic light scattering (DLS) and a zeta potential analyzer. The effects, including chitosan molecular weight, chitosan concentration, ammonium glycyrrhizinate concentration and polyethylene glycol (PEG) on the physicochemical properties of the nanoparticles were studied. These nanoparticles have ammonium glycyrrhizinate loading efficiency. The encapsulation efficiency decreased with the increase of ammonium glycyrrhizinate concentration and chitosan concentration. The introduction of PEG can decrease significantly the positive charge of particle surface. These studies showed that chitosan can complex TPP to form stable cationic nanoparticles for subsequent ammonium glycyrrhizinate loading.

摘要

通过壳聚糖与三聚磷酸根阴离子（TPP）的离子凝胶化作用制备了负载甘草酸铵的壳聚糖纳米颗粒。分别采用动态光散射（DLS）和zeta电位分析仪测定纳米颗粒的粒径和zeta电位。研究了壳聚糖分子量、壳聚糖浓度、甘草酸铵浓度和聚乙二醇（PEG）对纳米颗粒理化性质的影响。这些纳米颗粒具有甘草酸铵负载效率。包封率随甘草酸铵浓度和壳聚糖浓度的增加而降低。PEG的引入可显著降低颗粒表面的正电荷。这些研究表明，壳聚糖可与TPP络合形成稳定的阳离子纳米颗粒，用于后续负载甘草酸铵。