Efimova E A, Glanemann M, Nussler A K, Schumacher G, Settmacher U, Jonas S, Nussler N, Neuhaus P
Department of General-, Visceral- and Transplantation Surgery, Charité, Campus Virchow Clinic, University Medicine, Berlin, Germany.
Transplant Proc. 2005 Mar;37(2):1074-5. doi: 10.1016/j.transproceed.2004.12.170.
To obtain better insight into the kinetics of hepatic growth factors following partial hepatectomy for living related liver donation, we investigated the postoperative changes in serum levels of hepatocyte growth factor (HGF), epidermal growth factor (EGF), vascular epidermal growth factor (VEGF), and transforming growth factor-alpha (TGF-alpha).
Eighteen healthy donors undergoing right hepatectomy for living related donation were enrolled in this study. Serum levels of HGF, EGF, VEGF, and TGF-alpha were measured using enzyme-linked immunosorbent assay kits before surgery, at 2 hours after resection, and daily during 5 days postoperatively.
Mean preoperative HGF serum levels in healthy adults were 778 +/- 64 pg/mL. Within 2 hours after operation, they significantly increased to 9608 +/- 3111 pg/mL afterward decreasing to 2726 +/- 241 at day 1 and 2283 +/- 250 pg/mL at day 2. Hereafter HGF serum levels stabilized at increased levels until day 5 (2109 +/- 138, 2047 +/- 219, 2283 +/- 336 pg/mL, respectively). At all time points, the differences between pre- and postoperative HGF levels were significant (P < .01). In contrast, VEGF and EGF serum levels showed no significant differences between pre- and postoperative levels at all time points. TGF-alpha was not detected using a commercially available test with a detection limit of 10 ng/mL, suggesting only low TGF-alpha serum levels following liver resection.
Significantly increased HGF serum levels after hepatectomy demonstrate its crucial role among the other investigated growth factors in regeneration of the remnant liver tissue during the early period after the operation.
为了更深入了解活体亲属肝移植部分肝切除术后肝生长因子的动力学，我们研究了肝细胞生长因子（HGF）、表皮生长因子（EGF）、血管内皮生长因子（VEGF）和转化生长因子-α（TGF-α）血清水平的术后变化。
本研究纳入了18例接受活体亲属右肝切除术的健康供体。术前、切除后2小时以及术后5天每天使用酶联免疫吸附测定试剂盒测量HGF、EGF、VEGF和TGF-α的血清水平。
健康成年人术前HGF血清平均水平为778±64 pg/mL。术后2小时内，其显著升高至9608±3111 pg/mL，随后在第1天降至2726±241 pg/mL，第2天降至2283±250 pg/mL。此后，HGF血清水平在升高状态下稳定至第5天（分别为2109±138、2047±219、2283±336 pg/mL）。在所有时间点，术前和术后HGF水平之间的差异均具有统计学意义（P <.01）。相比之下，VEGF和EGF血清水平在所有时间点的术前和术后水平之间均无显著差异。使用检测限为10 ng/mL的市售检测方法未检测到TGF-α，提示肝切除术后TGF-α血清水平较低。
肝切除术后HGF血清水平显著升高，表明其在术后早期残余肝组织再生的其他研究生长因子中起关键作用。
