Kaufman L J, Brangwynne C P, Kasza K E, Filippidi E, Gordon V D, Deisboeck T S, Weitz D A
Division of Engineering and Applied Sciences, and Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
Biophys J. 2005 Jul;89(1):635-50. doi: 10.1529/biophysj.105.061994. Epub 2005 Apr 22.
We study the growth and invasion of glioblastoma multiforme (GBM) in three-dimensional collagen I matrices of varying collagen concentration. Phase-contrast microscopy studies of the entire GBM system show that invasiveness at early times is limited by available collagen fibers. At early times, high collagen concentration correlates with more effective invasion. Conversely, high collagen concentration correlates with inhibition in the growth of the central portion of GBM, the multicellular tumor spheroid. Analysis of confocal reflectance images of the collagen matrices quantifies how the collagen matrices differ as a function of concentration. Studying invasion on the length scale of individual invading cells with a combination of confocal and coherent anti-Stokes Raman scattering microscopy reveals that the invasive GBM cells rely heavily on cell-matrix interactions during invasion and remodeling.
我们研究了多形性胶质母细胞瘤（GBM）在不同胶原蛋白浓度的三维I型胶原蛋白基质中的生长和侵袭情况。对整个GBM系统的相差显微镜研究表明，早期的侵袭性受到可用胶原纤维的限制。在早期，高胶原蛋白浓度与更有效的侵袭相关。相反，高胶原蛋白浓度与GBM中央部分即多细胞肿瘤球体的生长抑制相关。对胶原蛋白基质的共聚焦反射图像分析量化了胶原蛋白基质如何随浓度变化而不同。结合共聚焦显微镜和相干反斯托克斯拉曼散射显微镜，在单个侵袭细胞的长度尺度上研究侵袭情况，结果表明侵袭性GBM细胞在侵袭和重塑过程中严重依赖细胞与基质的相互作用。
