Kato Masashi
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Biosci Biotechnol Biochem. 2005 Apr;69(4):663-72. doi: 10.1271/bbb.69.663.
Filamentous fungi are frequently used for the production of industrial enzymes, since they produce a variety of enzymes including polysaccharide-degrading enzymes. Among the many filamentous fungi, Aspergillus species, such as A. oryzae and A. niger, are known as strong producers of amylolytic enzymes. We have been studying on the regulatory mechanisms underlying the expression of A. oryzae amylolytic genes. Based on analyses using a hybrid model system of A. nidulans transformed by a gene encoding A. oryzae Taka-amylase A, the major amylase (taaG2), we have found that three factors, CCAAT-box binding protein, CreA, and AmyR, are involved in taaG2 gene expression and regulation. In this review, the focus is on the CCAAT-box binding protein of filamentous fungi. The assembly, nuclear translocation, and transcriptional enhancement mechanisms of the CCAAT-box binding protein are discussed.
丝状真菌常用于工业酶的生产，因为它们能产生多种酶，包括多糖降解酶。在众多丝状真菌中，米曲霉和黑曲霉等曲霉属物种是已知的淀粉酶高产菌株。我们一直在研究米曲霉淀粉酶基因表达的调控机制。基于使用编码米曲霉Taka淀粉酶A（主要淀粉酶taaG2）的基因转化的构巢曲霉杂交模型系统进行的分析，我们发现CCAAT框结合蛋白、CreA和AmyR这三个因子参与taaG2基因的表达和调控。在这篇综述中，重点是丝状真菌的CCAAT框结合蛋白。讨论了CCAAT框结合蛋白的组装、核转位和转录增强机制。
