Boaz Mona, Iuliano Luigi, Himmelfarb Jonathan, Matas Zipora, Micheletta Fausta, McMonagle Ellen, Friedman Victoria, Natoli Silvia, Gvirtz Gabriella, Biro Alexander, Smetana Shmuel, Sabo Gideon, Gafter Uzi, Weinstein Talia
Epidemiology Unit, E. Wolfson Medical Center, Holon, Israel.
Nephron Clin Pract. 2005;100(4):c111-9. doi: 10.1159/000085290. Epub 2005 Apr 20.
Oxysterols are markers of oxidative stress, levels of which have not yet been reported in hemodialysis (HD) patients. This study was designed to compare levels of the oxysterols 7-ketocholesterol (7KC) and 7beta-hydroxycholesterol (7betaOH) between a cohort of HD patients and healthy controls.
This nested cross-sectional study reflects baseline (pre-intervention) values for markers of oxidative stress, inflammation and nutrition status in the 160-member vitamin E and carotid intima media thickness progression in end-stage renal disease (VIPER) cohort (age 64.1 +/- 8.8, 33.5% female). Age- and sex-matched healthy volunteers served as controls. Plasma oxysterols 7KC and 7betaOH were determined by isotope dilution gas chromatography/mass spectrometry.
Despite higher plasma alpha-tocopherol levels in HD patients than controls (36.0 +/- 9.3 vs. 31.8 +/- 8.4 micromol/l, p = 0.007), 7KC levels (9.8 +/- 6.9 vs. 5.9 +/- 2.8 nmol/mmol cholesterol, p < 0.0001) and 7betaOH levels (8.7 +/- 4.3 vs. 2.7 +/- 1.6 nmol/mmol cholesterol, p < 0.0001) were higher in HD patients. The oxysterol 7betaOH was significantly, inversely associated with prealbumin (r = -0.18, p = 0.03), though neither oxysterol was significantly associated with any other marker of oxidative stress, inflammation or nutrition status and did not discriminate for CVD in HD patients.
Elevated levels of the oxysterols 7KC and 7betaOH indicate that HD patients are in a state of oxidative stress compared to healthy controls. However, oxysterols 7KC and 7betaOH did not appear to contribute additional information about oxidative stress among HD patients.
氧化甾醇是氧化应激的标志物，血液透析（HD）患者的氧化甾醇水平尚未见报道。本研究旨在比较一组HD患者与健康对照者的氧化甾醇7-酮胆固醇（7KC）和7β-羟基胆固醇（7βOH）水平。
这项嵌套横断面研究反映了160名成员的终末期肾病维生素E与颈动脉内膜中层厚度进展（VIPER）队列（年龄64.1±8.8岁，女性占33.5%）中氧化应激、炎症和营养状况标志物的基线（干预前）值。年龄和性别匹配的健康志愿者作为对照。血浆氧化甾醇7KC和7βOH通过同位素稀释气相色谱/质谱法测定。
尽管HD患者血浆α-生育酚水平高于对照组（36.0±9.3对31.8±8.4μmol/L，p = 0.007），但HD患者的7KC水平（9.8±6.9对5.9±2.8 nmol/mmol胆固醇，p < 0.0001）和7βOH水平（8.7±4.3对2.7±1.6 nmol/mmol胆固醇，p < 0.0001）更高。氧化甾醇7βOH与前白蛋白显著负相关（r = -0.18，p = 0.03），不过两种氧化甾醇均与氧化应激、炎症或营养状况的任何其他标志物无显著相关性，且在HD患者中对心血管疾病无鉴别作用。
氧化甾醇7KC和7βOH水平升高表明，与健康对照者相比，HD患者处于氧化应激状态。然而，氧化甾醇7KC和7βOH似乎并未为HD患者氧化应激提供更多信息。
