Hodkova Magdalena, Dusilova-Sulkova Sylvie, Kalousova Marta, Soukupova Jirina, Zima Tomas, Mikova Dana, Malbohan Ivan Matous, Bartunkova Jirina
Department of Nephrology, Institute of Clinical Chemistry and Laboratory Diagnostics, General Teaching Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic.
Ren Fail. 2006;28(5):395-9. doi: 10.1080/08860220600683698.
The aim of this study was to evaluate the influence of oral vitamin E therapy on serum concentrations of several markers of micro-inflammation and cardiovascular disease in chronic hemodialysis (HD) patients.
29 HD patients were randomized into two groups: 15 patients were treated orally with 400 mg of vitamin E daily for a period of five weeks, and 14 patients received no antioxidant supplementation. Before and after vitamin E therapy, serum concentrations of vitamin E (high-performance liquid chromatography), pregnancy-associated plasma protein-A (immunochemical--TRACE assay), C-reactive protein (nephelometry), intercellular adhesion molecule-1 (ELISA), and E-selectin (ELISA) were measured. HD patients were compared with 16 healthy controls.
Baseline serum concentrations of PAPP-A and CRP were significantly higher in HD patients than in healthy controls (PAPP-A: 26.23+/-11.94 vs. 11.41+/-1.94 mIU/L, p<0.001; CRP: 5.20+/-3.50 vs. 3.40+/-3.80 mg/L, p<0.05). After five weeks of oral vitamin E intake, serum PAPP-A, CRP, ICAM-1, and E-selectin concentrations remained unchanged in both groups of HD patients.
Chronic micro-inflammation in HD patients is documented by the elevation of CRP and PAPP-A. A daily oral dose of 400 mg of vitamin E does not seem to be able to reduce enhanced oxidative stress and micro-inflammation in chronic HD patients.
本研究旨在评估口服维生素E疗法对慢性血液透析（HD）患者血清中几种微炎症和心血管疾病标志物浓度的影响。
29例HD患者被随机分为两组：15例患者每天口服400毫克维生素E，持续五周，14例患者未接受抗氧化剂补充。在维生素E治疗前后，测量血清中维生素E（高效液相色谱法）、妊娠相关血浆蛋白-A（免疫化学-TRACE检测法）、C反应蛋白（散射比浊法）、细胞间黏附分子-1（酶联免疫吸附测定法）和E-选择素（酶联免疫吸附测定法）的浓度。将HD患者与16名健康对照者进行比较。
HD患者的PAPP-A和CRP基线血清浓度显著高于健康对照者（PAPP-A：26.23±11.94对11.41±1.94 mIU/L，p<0.001；CRP：5.20±3.50对3.40±3.80 mg/L，p<0.05）。口服维生素E五周后，两组HD患者的血清PAPP-A、CRP、ICAM-1和E-选择素浓度均未改变。
HD患者的慢性微炎症通过CRP和PAPP-A的升高得到证实。每日口服400毫克维生素E似乎无法降低慢性HD患者增强的氧化应激和微炎症。
