Iveković Damir, Milardović Stjepan, Roboz Mario, Grabarić Bozidar S
Laboratory of General and Inorganic Chemistry and Electroanalysis, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia.
Analyst. 2005 May;130(5):708-14. doi: 10.1039/b415939j. Epub 2005 Mar 15.
A Trolox equivalent antioxidant capacity (TEAC) decolourisation assay was adapted to a flow injection analysis (FIA) system and a simple and rapid method for antioxidant activity evaluation was developed. To avoid the time consuming step of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical cation preparation by chemical oxidation of ABTS, as in the original TAEC assay, and hence, to shorten the analysis time, the ABTS radical cation was generated on-line by electrochemical oxidation of ABTS in the flow-through electrolysis cell forming a part of the FIA system. The proposed method was optimised with respect to a flow rate, injection volume and ABTS radical cation/carrier ratio. Under the optimised conditions linear calibration graphs for Trolox were obtained over the range 10-100 microM, with a limit of detection 1.6 microM. Good reproducibility (relative standard deviation 1.95%) and sample throughput (32 samples per hour) were achieved. The developed method was applied to the evaluation of the antioxidant activity of pure compounds and samples of some common beverages. In both cases a good correlation between the results obtained by the proposed method and TEAC values evaluated by the classic TAEC decolourisation assay was obtained (r(2)= 0.996 for pure compounds and r(2)= 0.957 for beverage samples).
将一种Trolox等效抗氧化能力（TEAC）脱色测定法应用于流动注射分析（FIA）系统，开发了一种简单快速的抗氧化活性评估方法。为避免像原始TEAC测定法那样通过化学氧化2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）来制备ABTS自由基阳离子这一耗时步骤，从而缩短分析时间，ABTS自由基阳离子通过在构成FIA系统一部分的流通式电解池中对ABTS进行电化学氧化在线生成。该方法针对流速、进样体积和ABTS自由基阳离子/载体比例进行了优化。在优化条件下，获得了Trolox在10 - 100 microM范围内的线性校准曲线，检测限为1.6 microM。实现了良好的重现性（相对标准偏差1.95%）和样品通量（每小时32个样品）。所开发的方法应用于纯化合物和一些常见饮料样品的抗氧化活性评估。在这两种情况下，所提出方法获得的结果与经典TEAC脱色测定法评估的TEAC值之间都具有良好的相关性（纯化合物的r(2)= 0.996，饮料样品的r(2)= 0.957）。
