Qu Liangwei, Luo Pengju G, Taylor Shelby, Lin Yi, Huang Weijie, Anyadike Nkechinyere, Tzeng Tzuen-Rong J, Stutzenberger Fred, Latour Robert A, Sun Ya-Ping
Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA.
J Nanosci Nanotechnol. 2005 Feb;5(2):319-22. doi: 10.1166/jnn.2005.043.
Polymeric nanoparticles covalently functionalized with derivatized D-mannose molecules were synthesized and characterized. These nanoparticles have an average size of approximately 160 nm in diameter, thus bearing a large number of surface-tethered mannose moieties for multivalent interactions with adhesins on bacterial cells. Specifically, the mannosylated nanoparticles bind strongly with Escherichia coli, allowing the convenient visualization of adhesion interactions under a conventional electron microscope. Since a single nanoparticle is capable of binding more than one cell, the adhesion interactions result in significant nanoparticle-mediated cell agglutination according to electron microscopy imaging. Potential applications of the mannosylated nanoparticles in the inhibition of enteropathogenic infections are discussed.
合成并表征了用衍生化的D-甘露糖分子共价功能化的聚合物纳米颗粒。这些纳米颗粒的平均直径约为160nm，因此带有大量表面连接的甘露糖部分，可用于与细菌细胞上的粘附素进行多价相互作用。具体而言，甘露糖化纳米颗粒与大肠杆菌强烈结合，便于在传统电子显微镜下观察粘附相互作用。由于单个纳米颗粒能够结合多个细胞，根据电子显微镜成像，粘附相互作用会导致纳米颗粒介导的显著细胞凝集。讨论了甘露糖化纳米颗粒在抑制肠道致病性感染方面的潜在应用。
