Kitazawa Manabu, Ishitsuka Yukiko, Kobayashi Misako, Nakano Takashi, Iwasaki Keiji, Sakamoto Kazutami, Arakane Kumi, Suzuki Tadashi, Kligman Lorraine H
AminoScience Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Photochem Photobiol. 2005 Jul-Aug;81(4):970-4. doi: 10.1562/2004-09-18-RA-320.
The generation of reactive oxygen species (ROS) by ultraviolet radiation (UVR) accelerates skin aging, which is known as photoaging. Because cutaneous iron catalyzes ROS generation, sequestering iron by chelating agents is thought to be an effective approach toward preventing photoaging. Previously, N-(4-pyridoxylmethylene)-l-serine (PYSer) was designed as an antioxidant to suppress iron-catalyzed ROS generation by its iron-sequestering activity. In this study, PYSer showed protective effects against skin damage in hairless mice irradiated with ultraviolet B (UV-B). Topical application of PYSer to the skin significantly delayed and/or decreased the visible wrinkle formation induced by chronic UV-B irradiation. A histological study indicated that UV-B-induced epidermal hypertrophy and lymphocytic infiltration were suppressed by PYSer. Moreover, PYSer showed suppressive activity against the UV-B-induced increase in glycosaminoglycans (GAG). These results indicate that PYSer is a promising antioxidant for the prevention of chronic skin photoaging by its iron-sequestering activity.
紫外线辐射（UVR）产生活性氧（ROS）会加速皮肤衰老，即光老化。由于皮肤中的铁会催化ROS生成，因此通过螯合剂螯合铁被认为是预防光老化的有效方法。此前，N-(4-吡啶基亚甲基)-L-丝氨酸（PYSer）被设计为一种抗氧化剂，通过其铁螯合活性抑制铁催化的ROS生成。在本研究中，PYSer对紫外线B（UV-B）照射的无毛小鼠的皮肤损伤具有保护作用。将PYSer局部应用于皮肤可显著延迟和/或减少慢性UV-B照射引起的可见皱纹形成。组织学研究表明，PYSer可抑制UV-B诱导的表皮肥厚和淋巴细胞浸润。此外，PYSer对UV-B诱导的糖胺聚糖（GAG）增加具有抑制活性。这些结果表明，PYSer通过其铁螯合活性有望成为预防慢性皮肤光老化的抗氧化剂。
