Zhow Y, Zhu M, Cheng J, Yian B, Yiang Y
Apheresis Department, Nanjing Red Cross Blood Center, China.
Transfusion. 1992 May;32(4):349-53. doi: 10.1046/j.1537-2995.1992.32492263450.x.
A simplified technique has been developed to separate albumin from the plasma of a patient undergoing treatment with plasma exchange. This albumin can be returned to the patient to maintain colloid osmotic pressure without risk of transmitting disease. Patient plasma is obtained by apheresis. It is heated at 70 degrees C for 60 minutes to denature and remove autoantibodies, immune complexes, and abnormal globulins. The resulting low-concentration albumin solution (LCAS) retains its natural properties. Immunologic analysis by gel diffusion shows a reaction pattern of complete identity with that of human serum albumin. The albumin in LCAS is more than 90 percent of the remaining protein, as determined by scan of a cellulose acetate electrophoresis pattern. More than 97 percent of the albumin is in monomer form, as judged by polyacrylamide gel electrophoresis. No patient antibodies to neonantigens in the heat-treated albumin can be identified by double radial immunodiffusion. More than 80 percent of the plasma albumin is recovered in LCAS. LCAS is safe and efficient for use in plasma exchange.
已开发出一种简化技术，用于从接受血浆置换治疗的患者血浆中分离白蛋白。这种白蛋白可回输给患者以维持胶体渗透压，而无传播疾病的风险。患者血浆通过单采术获取。将其在70摄氏度加热60分钟，以使自身抗体、免疫复合物和异常球蛋白变性并去除。所得的低浓度白蛋白溶液（LCAS）保留了其天然特性。通过凝胶扩散进行的免疫学分析显示，其反应模式与人类血清白蛋白完全相同。通过醋酸纤维素电泳图谱扫描测定，LCAS中的白蛋白占剩余蛋白质的90%以上。通过聚丙烯酰胺凝胶电泳判断，超过97%的白蛋白为单体形式。通过双向放射免疫扩散无法鉴定出热处理白蛋白中针对新抗原的患者抗体。超过80%的血浆白蛋白在LCAS中得以回收。LCAS用于血浆置换安全且有效。
