通过在凝胶基质中加入蓝色葡聚糖，在天然聚丙烯酰胺凝胶电泳中血清白蛋白迁移的延迟。
Retardation of serum albumin migration in native polyacrylamide gel electrophoresis by the incorporation of blue dextran in the gel matrix.
作者信息
Pemberton A D, John H A
机构信息
Moredun Research Institute, Edinburgh, Midlothian, Scotland, UK.
出版信息
Electrophoresis. 1993 Mar;14(3):240-1. doi: 10.1002/elps.1150140140.
PMID:7683609
Abstract
Blue dextran was incorporated into the stacking layer of one-dimensional polyacrylamide gels to prevent the migration of albumin into the resolving gel during the electrophoresis of native plasma samples. Human and horse plasma proteins normally masked by albumin were revealed by this method.
摘要
在一维聚丙烯酰胺凝胶的堆积层中加入蓝色葡聚糖，以防止在天然血浆样品电泳过程中白蛋白迁移到分离胶中。通过这种方法揭示了通常被白蛋白掩盖的人和马的血浆蛋白。
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