Pinto Ligia A, Castle Philip E, Roden Richard B, Harro Clayton D, Lowy Douglas R, Schiller John T, Wallace Dora, Williams Marcus, Kopp William, Frazer Ian H, Berzofsky Jay A, Hildesheim Allan
SAIC-Frederick, Inc./NCI-Frederick, Room 120, Building 469, Frederick, MD 21702, USA.
Vaccine. 2005 May 20;23(27):3555-64. doi: 10.1016/j.vaccine.2005.01.146.
Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n=20) or placebo (n=4) before and after vaccination. 11 cytokines were measured: IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IFN-gamma, TNF-alpha, and GM-CSF using multiplex bead arrays. Cytokine profiles from WB samples clearly discriminated between vaccine and placebo recipients and between pre and post-vaccination responses. Significant increases in Th1, Th2 and inflammatory cytokines were observed in WB assays following vaccination. Results from WB assays were compared against parallel PBMC-based assays in a subset of patients. Differences between whole blood assay and PBMC were observed, with the highest levels of induction found for WB for several cytokines. Our results indicate that multiplex assays for cytokine profiling in WB are an efficient tool for assessing broad spectrum, innate and adaptive immune responses to vaccines and identifying immunologic correlates of protection in efficacy studies.
在此，我们评估了在接种疫苗前和接种后，来自接种疫苗者（n = 20）或安慰剂组（n = 4）的全血（WB）培养物中，HPV - 16 L1病毒样颗粒（VLP）诱导的先天性和适应性免疫系统细胞因子反应。使用多重微珠阵列检测了11种细胞因子：IL - 1β、IL - 2、IL - 4、IL - 5、IL - 6、IL - 8、IL - 10、IL - 12、IFN - γ、TNF - α和GM - CSF。WB样本的细胞因子谱能够清晰地区分疫苗接种者和安慰剂接受者，以及接种前和接种后的反应。接种疫苗后，在WB检测中观察到Th1、Th2和炎性细胞因子显著增加。在一部分患者中，将WB检测结果与基于外周血单个核细胞（PBMC）的平行检测结果进行了比较。观察到全血检测和PBMC检测之间存在差异，几种细胞因子在WB检测中的诱导水平最高。我们的结果表明，用于WB中细胞因子谱分析的多重检测是评估对疫苗的广谱先天性和适应性免疫反应以及在疗效研究中确定保护的免疫相关因素的有效工具。
