Bouts Antonia H M, Davin Jean-Claude, Krediet Raymond T, Schröder Cornelis H, Monnens Leo A H, Nauta Jeroen, van de Winkel Jan G J, Out Theo A
Emma Children's Hospital AMC, University of Amsterdam, The Netherlands.
Pediatr Nephrol. 2005 Aug;20(8):1161-7. doi: 10.1007/s00467-005-1850-8. Epub 2005 Apr 26.
Children treated by peritoneal dialysis (PD) are at increased risk of infections. IgG receptors (FcgammaRs) and complement receptors (CRs) on white blood cells (WBCs) are important for the phagocytic process. We have investigated FcgammaR and CR expression on monocytes, macrophages and neutrophils in blood and in peritoneal dialysis effluent (PDE) of 39 PD children. WBCs were isolated from blood and PDE, labelled with FITC-conjugated CD16 (FcgammaRIII), CD32 (FcgammaRII), CD64 (FcgammaRI), CD11b (CR3) and CD35 (CR1) monoclonal antibodies, and analysed by flow cytometry. Peritoneal cells had lower percentages of FcgammaR-positive or CR-positive cells than blood. On the other hand, the receptor number per cell [mean fluorescence intensity (MFI)] was higher on peritoneal macrophages and neutrophils than blood, except for CD16. The FcgammaR and CR expression in blood and dialysate did not change significantly during the first year of PD treatment. During a peritonitis episode the MFI of all receptors in blood increased only on monocytes, with the exception of CD32. The percentages of FcgammaR-positive and CR-positive macrophages and neutrophils in the PDE increased, whereas the MFI did not increase consistently. Peritoneal cells of PD children showed a lower percentage of FcgammaR-positive and CR-positive neutrophils and macrophages, combined with an increased MFI, indicating a state of activation. Blood and peritoneal cells are capable of up-regulating the receptor expression during peritonitis but probably not to a maximum level.
接受腹膜透析（PD）治疗的儿童感染风险增加。白细胞（WBC）上的IgG受体（FcγRs）和补体受体（CRs）对吞噬过程很重要。我们研究了39名接受PD治疗儿童的血液和腹膜透析流出液（PDE）中单核细胞、巨噬细胞和中性粒细胞上FcγR和CR的表达。从血液和PDE中分离白细胞，用异硫氰酸荧光素（FITC）偶联的CD16（FcγRIII）、CD32（FcγRII）、CD64（FcγRI）、CD11b（CR3）和CD35（CR1）单克隆抗体标记，并通过流式细胞术进行分析。腹膜细胞中FcγR阳性或CR阳性细胞的百分比低于血液。另一方面，除CD16外，腹膜巨噬细胞和中性粒细胞上每个细胞的受体数量[平均荧光强度（MFI）]高于血液。在PD治疗的第一年，血液和透析液中FcγR和CR的表达没有明显变化。在腹膜炎发作期间，血液中所有受体的MFI仅在单核细胞上增加，CD32除外。PDE中FcγR阳性和CR阳性巨噬细胞及中性粒细胞的百分比增加，而MFI并未持续增加。接受PD治疗儿童的腹膜细胞显示FcγR阳性和CR阳性中性粒细胞及巨噬细胞的百分比降低，同时MFI增加，表明处于激活状态。血液和腹膜细胞在腹膜炎期间能够上调受体表达，但可能未达到最高水平。
