Williams Vijai, Rawat Amit, Vignesh Pandiarajan, Shandilya Jitendra Kumar, Gupta Anju, Singh Surjit
Pediatric Allergy and Immunology Unit, Department of Pediatrics, Advanced Pediatrics Centre, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Int J Rheum Dis. 2019 Mar;22(3):449-457. doi: 10.1111/1756-185X.13403. Epub 2018 Nov 6.
Polymorphisms in the Fcγ-receptor (FcγR) have been associated with increased susceptibility to systemic lupus erythematosus (SLE). There is a paucity of data on FcγR expression pattern in pediatric subjects with SLE. The aim of the study was to assess the expression of various FcγRs by flow cytometry in children with pediatric-onset SLE (pSLE).
Thirty-one children aged 0-15 years fulfilling 2012 Systemic Lupus International Collaborating Clinics Classification Criteria for SLE were enrolled. Disease-active (n = 14) and the inactive group were delineated using the SLE Disease Activity Index (SLEDAI). Thirteen age- and sex-matched controls were also enrolled. Blood samples of cases and controls were assessed for CD64, CD32B and CD16 expression on B lymphocytes, neutrophils and monocytes by flow cytometry using standard techniques. Median fluorescence intensity (MFI) and percentage expression were calculated using the FACS DIVA software and Kaluza software.
Median fluorescence intensity and percentage expression of CD64 on monocytes (MFI: 1.71 vs 1.51, P = 0.86) and neutrophils (MFI: 0.42 vs 0.64, P = 0.3) were comparable between patients and controls. MFIs of CD16 expression on neutrophils (3.47 vs 11.4, P = 0.05) and monocytes (1.28 vs 3.45, P = 0.07) were lower in patients compared to controls. CD32B expression on lymphocytes (MFI: 0.56 vs 1.37; % expression:18.3% vs 12.32%) was also comparable between cases and controls. Expression of CD64, CD16, and CD32B were also comparable between patients with active and inactive disease.
No significant differences were observed in FcγR expression between patients and controls. However, the overall trends of FcγR expression and decreased CD16 on monocytes and neutrophils are in consonance with data from larger cohorts of adult SLE patients.
Fcγ受体（FcγR）多态性与系统性红斑狼疮（SLE）易感性增加有关。关于儿童SLE患者FcγR表达模式的数据较少。本研究的目的是通过流式细胞术评估儿童期发病的SLE（pSLE）患儿中各种FcγR的表达。
纳入31例年龄在0至15岁、符合2012年系统性红斑狼疮国际协作临床分类标准的SLE患儿。使用SLE疾病活动指数（SLEDAI）划分疾病活动组（n = 14）和非活动组。还纳入了13例年龄和性别匹配的对照。采用标准技术通过流式细胞术评估病例组和对照组血液样本中B淋巴细胞、中性粒细胞和单核细胞上CD64、CD32B和CD16的表达。使用FACS DIVA软件和Kaluza软件计算中位荧光强度（MFI）和表达百分比。
患者和对照组单核细胞上CD64的中位荧光强度和表达百分比（MFI：1.71对1.51，P = 0.86）以及中性粒细胞上CD64的中位荧光强度和表达百分比（MFI：0.42对0.64，P = 0.3）相当。与对照组相比，患者中性粒细胞（3.47对11.4，P = 0.05）和单核细胞（1.28对3.45，P = 0.07）上CD16表达的MFI较低。病例组和对照组淋巴细胞上CD32B的表达（MFI：0.56对1.37；表达百分比：18.3%对12.32%）也相当。疾病活动和非活动患者之间CD64、CD16和CD32B的表达也相当。
患者和对照组之间FcγR表达未观察到显著差异。然而，FcγR表达的总体趋势以及单核细胞和中性粒细胞上CD16表达降低与来自更大规模成年SLE患者队列的数据一致。
