一种来自褐藻施罗德氏团扇藻的木糖半乳岩藻聚糖可刺激内皮细胞合成抗血栓形成的硫酸乙酰肝素。

A xylogalactofucan from the brown seaweed Spatoglossum schröederi stimulates the synthesis of an antithrombotic heparan sulfate from endothelial cells.

作者信息

Rocha Hugo Alexandre de Oliveira, Bezerra Luana Cristina Lins de Medeiros, de Albuquerque Ivan Rui Lopes, Costa Leandro Silva, Guerra Celina Maria Pinto, de Abreu Luiz Diz, Nader Helena Bonciani, Leite Edda Lisboa

机构信息

Laboratório de Glicobiologia, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Rio Grande do Norte-UFRN, Natal-RN, Brazil.

出版信息

Planta Med. 2005 Apr;71(4):379-81. doi: 10.1055/s-2005-864110.

DOI:10.1055/s-2005-864110

PMID:15856421

Abstract

The brown seaweed Spatoglossum schröederi (Dictyotaceae) contains three main fucans (fucans A, B and C) with different mobility in electrophoresis. The fucan with highest mobility (fucan C) was precipitated with 2.0 volumes of acetone, purified using a combination of ion exchange chromatography and electrophoresis. It showed an MW of 24 kDa determined by HPLC and Sephadex G-75 chromatography and migrates as a single band in three distinct electrophoretic systems. This fucan contains fucose, xylose, galactose and sulfate in a molar ratio 1 : 0.6 : 2:2.3. The fucan has neither anticoagulant (from 10 to 100 microg) nor hemorrhagic activities (100 microg/mL). In addition, fucan C is neither cytotoxic nor cytostatic. However, fucan C (100 microg/mL) stimulated the synthesis of an antithrombotic heparan sulfate from endothelial cells of rabbit aorta. The results suggest that fucan C might be used as an antithrombotic therapeutic compound.

摘要

棕色海藻施氏网翼藻（网地藻科）含有三种主要的岩藻聚糖（岩藻聚糖A、B和C），它们在电泳中的迁移率不同。迁移率最高的岩藻聚糖（岩藻聚糖C）用2.0倍体积的丙酮沉淀，通过离子交换色谱和电泳相结合的方法进行纯化。通过高效液相色谱和葡聚糖凝胶G-75色谱法测定，其分子量为24 kDa，并且在三种不同的电泳系统中均以单一条带迁移。这种岩藻聚糖中岩藻糖、木糖、半乳糖和硫酸盐的摩尔比为1:0.6:2:2.3。该岩藻聚糖既没有抗凝活性（10至100微克），也没有出血活性（100微克/毫升）。此外，岩藻聚糖C既没有细胞毒性也没有细胞抑制作用。然而，岩藻聚糖C（100微克/毫升）刺激了兔主动脉内皮细胞合成抗血栓形成的硫酸乙酰肝素。结果表明，岩藻聚糖C可能用作抗血栓治疗化合物。