Yokoyama Eiji, Shibusawa Yayoi, Maruyama Soichi, Katsube Yasuji, Mikami Takeshi
Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2, Nitona, Chuo, Chiba City, Chiba 260-8715, Japan.
Comp Immunol Microbiol Infect Dis. 2005 May;28(3):177-86. doi: 10.1016/j.cimid.2005.01.002.
Inhibition of isolation of Listeria monocytogenes by bacteriocin-like substance (BLS)-producing Listeria innocua after enrichment culture was investigated. When 26 L. monocytogenes strains were examined in combination with eight L. innocua strains using the spot on lawn method, 52/208 (25.0%) combinations showed the growth inhibition of L. monocytogenes. When two Listeria species were cultured simultaneously in selective enrichment broth, inhibition of isolation of L. monocytogenes was observed in 12/52 of the combinations at 24h (23.1%), in 24/52 at 48h (46.2%) and in 30/52 (57.7%) after 7 days of incubation. The randomly amplified polymorphic DNA profiles showed no interstrain similarities between either strains of the BLS-producing L. innocua or the BLS-sensitive L. monocytogenes strains. Therefore inhibition by BLS-producing L. innocua of isolation of L. monocytogenes after enrichment culture is unlikely to be dependent upon a particular genetic profile.
研究了产类细菌素物质（BLS）的无害李斯特菌在富集培养后对单核细胞增生李斯特菌分离的抑制作用。当使用菌苔点种法将26株单核细胞增生李斯特菌菌株与8株无害李斯特菌菌株组合检测时，52/208（25.0%）的组合显示出对单核细胞增生李斯特菌生长的抑制作用。当两种李斯特菌在选择性富集肉汤中同时培养时，在培养24小时后，12/52（23.1%）的组合中观察到对单核细胞增生李斯特菌分离的抑制，在48小时时为24/52（46.2%），在培养7天后为30/52（57.7%）。随机扩增多态性DNA图谱显示，产BLS的无害李斯特菌菌株或对BLS敏感的单核细胞增生李斯特菌菌株之间均无菌株间相似性。因此，产BLS的无害李斯特菌在富集培养后对单核细胞增生李斯特菌分离的抑制作用不太可能依赖于特定的基因图谱。
