Yokoyama Eiji, Saitoh Tetsuji, Maruyama Soichi, Katsube Yasuji
Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2, Nitona, Chuo, Chiba City, Chiba 260-8715, Japan.
Comp Immunol Microbiol Infect Dis. 2005 Jul;28(4):259-68. doi: 10.1016/j.cimid.2005.03.002. Epub 2005 Jun 1.
The actual prevalence of Listeria monocytogenes from contents of swine cecum was investigated. The efficiency of Listeria enrichment broth (LEB) for isolation was examined by the recovery of artificially inoculated L. monocytogenes in contents of swine cecum. The numbers of organisms did not increase after 48 h incubation, but increased when the rapid decrease in pH of the LEB was adjusted. Between 1991 and 1993, 250 contents of swine cecum were examined for the prevalence of L. monocytogenes using LEB enrichment, either with or without pH adjustment. L. monocytogenes was isolated from 74 samples in 1993 with pH adjustment, however, no organisms were isolated in 1991 and 1992. It was suggested that the marked rise of the L. monocytogenes isolation was due to the spread of the organism among swine. Furthermore, 67 out of the 74 isolates were identified as 1/2c by serotyping. The serovar 1/2c strains showed genetic diversity by random amplified polymorphic DNA.
对猪盲肠内容物中单核细胞增生李斯特菌的实际流行情况进行了调查。通过在猪盲肠内容物中回收人工接种的单核细胞增生李斯特菌，检测了李斯特菌增菌肉汤（LEB）的分离效率。培养48小时后菌数未增加，但当调整LEB的pH值快速下降时菌数增加。1991年至1993年期间，使用LEB增菌法（无论是否调整pH值）对250份猪盲肠内容物进行了单核细胞增生李斯特菌流行情况的检测。1993年，在调整pH值的情况下，从74份样本中分离出了单核细胞增生李斯特菌，然而，1991年和1992年未分离到该菌。提示单核细胞增生李斯特菌分离率的显著上升是由于该菌在猪群中的传播。此外，通过血清分型，74株分离株中有67株被鉴定为1/2c型。1/2c血清型菌株通过随机扩增多态性DNA显示出遗传多样性。
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