Ewart K Vanya, Belanger Jasmine C, Williams Jason, Karakach Tobias, Penny Susanne, Tsoi Stephen C M, Richards Robert C, Douglas Susan E
Institute for Marine Biosciences, Halifax, NS, Canada B3H 3Z1.
Dev Comp Immunol. 2005;29(4):333-47. doi: 10.1016/j.dci.2004.08.004.
The response of Atlantic salmon, Salmo salar, to infection by the bacterial pathogen Aeromonas salmonicida (the causative agent of furunculosis), was investigated using a cohabitation model and a custom Atlantic salmon cDNA microarray consisting of over 4000 different amplicons. Pooled samples of each of three immune-relevant tissues (spleen, head kidney and liver) were obtained from fish exposed to infected salmon for 13 days. Reverse transcription-PCR assays were used to verify the differential expression of 12 candidate genes uncovered by microarray analysis. Among the differentially expressed genes were several previously revealed by suppression subtractive hybridization and EST surveys and that are recognized to encode humoral components of the innate immune system. Other genes identified in this study were not previously associated with infection. In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity.
利用同居模型和一个由4000多个不同扩增子组成的定制大西洋鲑鱼cDNA微阵列，研究了大西洋鲑（Salmo salar）对细菌性病原体杀鲑气单胞菌（疖疮病的病原体）感染的反应。从接触感染鲑鱼13天的鱼中获取了三种免疫相关组织（脾脏、头肾和肝脏）的混合样本。采用逆转录聚合酶链反应（RT-PCR）分析来验证微阵列分析发现的12个候选基因的差异表达。差异表达基因中包括一些先前通过抑制性消减杂交和EST调查揭示的、被认为编码先天免疫系统体液成分的基因。本研究中鉴定的其他基因以前与感染无关。此外，还发现了一些没有已知同源物的基因。确定它们在感染过程中的具体作用可能有助于更好地理解先天免疫。
