Gauthier Olivier, Müller Ralph, von Stechow Dietrich, Lamy Bernard, Weiss Pierre, Bouler Jean-Michel, Aguado Eric, Daculsi Guy
Ecole Nationale Vétérinaire de Nantes, Nantes, France.
Biomaterials. 2005 Sep;26(27):5444-53. doi: 10.1016/j.biomaterials.2005.01.072.
This in vivo study investigated the efficiency of an injectable calcium phosphate bone substitute (IBS) for bone regenerative procedures through non-destructive three-dimensional (3D) micro-tomographic (microCT) imaging, biomechanical testing with a non-destructive micro-indentation technique and 2D scanning electron microscopy (SEM) analysis. The injectable biomaterial was obtained by mixing a biphasic calcium phosphate (BCP) ceramic mineral phase and a cellulosic polymer. The BCP particles were 200-500 microm or 80-200 microm in diameter. The injectable material was implanted for 6 weeks into critical-sized bone defects at the distal end of rabbit femurs. Extensive new bone apposition was noted with both 2D and 3D techniques. Micro-CT showed that newly formed bone was in perfect continuity with the trabecular host bone structure and demonstrated the high interconnectivity of the restored bone network. For both IBS formulations, SEM and microCT gave very close measurements. The only detected significant difference concerned the amount of newly formed bone obtained with IBS 80-200 that appeared significantly higher with microCT analysis than with SEM (p=0.00007). Student t-tests did not show any significant difference in the amount of newly formed bone and remaining ceramic obtained from microCT analysis or SEM. Regression analysis showed satisfactory correlation between both the amount of newly formed bone and remaining ceramic obtained from microCT or SEM. For IBS 200-500, the newly formed bone rate inside the defect was 28.0+/-5.2% with SEM and yield strength of the samples was 18.8+/-5.4 MPa. For IBS 80-200, the newly formed bone rate inside the defect was 31.7+/-5.1% with SEM and yield strength of the samples was 26.8+/-4.5 MPa. Yield strength appeared well correlated with the amount of newly formed bone, specially observed with microCT. This study showed the ability of non-destructive techniques to investigate biological and mechanical aspects of bone replacement with injectable biomaterials.
本体内研究通过非破坏性三维（3D）显微断层扫描（microCT）成像、采用非破坏性微压痕技术的生物力学测试以及二维扫描电子显微镜（SEM）分析，研究了一种可注射磷酸钙骨替代物（IBS）用于骨再生手术的效率。这种可注射生物材料是通过将双相磷酸钙（BCP）陶瓷矿物相和纤维素聚合物混合而获得的。BCP颗粒的直径为200 - 500微米或80 - 200微米。将可注射材料植入兔股骨远端的临界尺寸骨缺损处6周。二维和三维技术均显示有广泛的新骨附着。MicroCT显示新形成的骨与小梁宿主骨结构完美连续，并证明了修复骨网络的高连通性。对于两种IBS配方，SEM和microCT给出的测量结果非常接近。唯一检测到的显著差异涉及用IBS 80 - 200获得的新形成骨的量，microCT分析显示其明显高于SEM（p = 0.00007）。学生t检验未显示从microCT分析或SEM获得的新形成骨的量和剩余陶瓷量有任何显著差异。回归分析表明，从microCT或SEM获得的新形成骨的量和剩余陶瓷量之间具有良好的相关性。对于IBS 200 - 500，SEM显示缺损内新形成骨率为28.0±5.2%，样品的屈服强度为18.8±5.4 MPa。对于IBS 80 - 200，SEM显示缺损内新形成骨率为31.7±5.1%，样品的屈服强度为26.8±4.5 MPa。屈服强度似乎与新形成骨的量密切相关，在microCT观察中尤为明显。本研究表明了非破坏性技术在研究可注射生物材料骨替代的生物学和力学方面的能力。
