Bernard Louis, Vaudaux Pierre, Merle Corinne, Stern Richard, Huggler Elzbieta, Lew Daniel, Hoffmeyer Pierre
Division of Orthopaedic Surgery, University Hospital of Geneva, Geneva, Switzerland.
Biomaterials. 2005 Sep;26(27):5552-7. doi: 10.1016/j.biomaterials.2004.08.034.
Following infection, bacterial killing by polymorphonuclear leukocytes (neutrophils) is the main host defense against bacteria. Our hypothesis is that particles of ultra-high molecular weight polyethylene (UHMWP) may impair local neutrophil function and consequently reduce neutrophil bacterial killing. To determine how the in vitro phagocytic-bactericidal activity of neutrophils was affected by exposure to wear particles, tests were run comparing the effects of different particle composition, and different concentrations and sizes of UHMWP particles. There was a significant correlation between the number of particles and the decrease in neutrophil bactericidal activity (p<0.01), and the greatest effect was obtained with a concentration of 10(7) UHMWP/ml. There was a significant decrease in neutrophil bactericidal activity by incubation with particles of 0.1-5 microm (p<0.01), but not with larger size. The results suggest that neutrophil functional defects triggered by the presence of UHMWP particles may potentially contribute to the susceptibility of loose implants to bacterial infections.
感染后，多形核白细胞（中性粒细胞）对细菌的杀伤作用是宿主抵御细菌的主要防御机制。我们的假设是，超高分子量聚乙烯（UHMWP）颗粒可能会损害局部中性粒细胞功能，从而降低中性粒细胞对细菌的杀伤能力。为了确定中性粒细胞的体外吞噬杀菌活性如何受到磨损颗粒暴露的影响，我们进行了测试，比较了不同颗粒组成、不同浓度和尺寸的UHMWP颗粒的影响。颗粒数量与中性粒细胞杀菌活性的降低之间存在显著相关性（p<0.01），当浓度为10(7) UHMWP/ml时效果最为明显。与0.1 - 5微米的颗粒孵育后，中性粒细胞杀菌活性显著降低（p<0.01），但与较大尺寸颗粒孵育则无此现象。结果表明，UHMWP颗粒的存在引发的中性粒细胞功能缺陷可能会导致松动植入物易发生细菌感染。
