Siriwattanarungsee Sirisuda, Sukontason Kabkaew L, Kuntalue Budsabong, Piangjai Somsak, Olson Jimmy K, Sukontason Kom
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Parasitol Res. 2005 Jun;96(3):166-70. doi: 10.1007/s00436-005-1343-5. Epub 2005 Apr 28.
Examination of the puparia of the housefly, Musca domestica L. and blowfly Chrysomya megacephala (F.), through scanning electron microscopy (SEM), revealed many differences in the profile of their morphology. Special attention was focused on puparial characteristics used to differentiate between the two fly species studied, and between other forensically important flies. Results of this study indicate that the housefly puparia are almost evenly rounded at both ends and the anterior spiracle bears six papillae. A pair of pupal respiratory horns is found laterally before the posterior boundary of the first abdominal segment, bearing numerous papillae that have a longitudinal opening along the oval convex base. The peritreme of each posterior spiracle forms a crude forward or reverse D-shape, encircling three sinuous slits. The blowfly pupariums anterior spiracle contains 8-12 papillae. The pupal respiratory horns protrude slightly and in some specimens a group of approximately 38 globules on the bubble-like membrane may be observed. Each of the posterior spiracles is more or less an oval- shaped peritreme, encircling three straight spiracular slits. The anatomical features presented herein allow for the differentiation of puparia of the two fly species studied and could prove useful in future forensic entomological assessments.
通过扫描电子显微镜（SEM）对家蝇（Musca domestica L.）和大头金蝇（Chrysomya megacephala (F.)）的蛹进行检查，发现它们的形态轮廓存在许多差异。特别关注用于区分所研究的两种蝇类以及其他法医学上重要蝇类的蛹特征。本研究结果表明，家蝇蛹的两端几乎均匀圆润，前气门有六个乳头。在第一腹节的后边界之前侧面发现一对蛹呼吸角，其上有许多乳头，沿着椭圆形凸基部有纵向开口。每个后气门的气门环形成一个粗糙的向前或向后的D形，环绕着三个弯曲的裂缝。大头金蝇蛹的前气门有8 - 12个乳头。蛹呼吸角略微突出，在一些标本中，可能会在气泡状膜上观察到一组约38个小球。每个后气门或多或少是椭圆形的气门环，环绕着三个直的气门裂缝。本文呈现的解剖特征有助于区分所研究的两种蝇类的蛹，并且可能在未来的法医昆虫学评估中证明是有用的。
