Bourel B, Fleurisse L, Hédouin V, Cailliez J C, Creusy C, Gosset D, Goff M L
Laboratoire Environment et Santé, Faculté Libre des Sciences et Faculté Libre de Médecine, Lille, France.
J Forensic Sci. 2001 May;46(3):596-9.
Morphine was detected by immunohistochemistry on sections of third stage larvae of Calliphora vomitoria (Diptera, Calliphoridae) reared on minced beef meat previously treated with morphine hydrochloride. The detection was performed with an avidin-biotin-peroxidase-complex method. Positive specimens showed specific staining of the haemolymph and a more intense immunoreaction in an area located at the limit between exocuticle and endocuticle. These results constitute an evidence of morphine accumulation inside the cuticle of Diptera larvae during their development. During the pupariation, the larval cuticle is transformed into the sclerotized puparium. This study consequently points out the possibilities of analyzing empty pupariae when suitable tissues or living necrophagous insects are absent.
在用盐酸吗啡预处理过的碎牛肉上饲养的红头丽蝇（双翅目，丽蝇科）第三期幼虫切片中，通过免疫组织化学检测到了吗啡。检测采用抗生物素蛋白-生物素-过氧化物酶复合物法进行。阳性标本显示血淋巴有特异性染色，在外皮和内表皮之间的界限区域有更强的免疫反应。这些结果证明了双翅目幼虫在发育过程中吗啡在其表皮内的积累。在化蛹过程中，幼虫表皮转化为硬化的蛹壳。因此，这项研究指出了在没有合适组织或活体食尸性昆虫时分析空蛹壳的可能性。
