Division of Pharmaceutical Sciences, Faculty of Pharmacy, Thammasat University, Pathum Thani, Thailand.
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Sci Rep. 2023 Sep 21;13(1):15689. doi: 10.1038/s41598-023-42500-8.
Musca domestica and Chrysomya megacephala, considered synanthropic insects, are medically important flies, as they transmit vector-borne diseases to humans and animals. In Thailand, Stemona (Stemonaceae) plants have been traditionally used as insecticides. This study was designed to determine the larvicidal activity of S. collinsiae root extract against M. domestica and C. megacephala larvae. A 70% ethanol crude extract from S. collinsiae roots was tested against the third-instar larvae of both species using direct and indirect contact methods. The development and mortality rates of the insects were observed, and the LC values were calculated. The extract caused irregular development in both species, shown as segmental puparia that could not emerge as adult flies. The LC values of the extract against M. domestica tested by direct and indirect contact methods were 0.0064 ± 0.0005 mg/larva and 0.0165 ± 0.0002 mg/cm/larva, respectively. In the case of C. megacephala, the LC value determined by the indirect contact method was 1.0500 ± 0.0001 mg/cm/larva. The ethanolic root extract of S. collinsiae was able to kill the larvae of both species after dermal administration. It is of interest to develop S. collinsiae root extract as a natural fly control biopesticide.
家蝇和大头金蝇被认为是共生昆虫，它们是重要的医学蝇类，因为它们会将病媒传播的疾病传播给人类和动物。在泰国，百部（百部科）植物已被传统用作杀虫剂。本研究旨在确定百部根提取物对家蝇和大头金蝇幼虫的杀幼虫活性。使用直接和间接接触方法，用 70%乙醇粗提物测试了百部根提取物对两种昆虫的三龄幼虫的活性。观察昆虫的发育和死亡率，并计算 LC 值。提取物对两种昆虫的发育都造成了不规则的影响，表现为不能变态为成蝇的节段性蛹。通过直接和间接接触方法测试的提取物对家蝇的 LC 值分别为 0.0064 ± 0.0005 mg/幼虫和 0.0165 ± 0.0002 mg/cm/幼虫。对于大头金蝇，间接接触法测定的 LC 值为 1.0500 ± 0.0001 mg/cm/幼虫。百部根的乙醇提取物在经皮给药后能够杀死两种幼虫。因此，开发百部根提取物作为天然蝇类控制生物农药具有一定的意义。
