Bartlett J M, Rabiasz G J, Scott W N, Langdon S P, Smyth J F, Miller W R
ICRF Medical Oncology Unit, Western General Hospital, Edinburgh, UK.
Br J Cancer. 1992 May;65(5):655-60. doi: 10.1038/bjc.1992.140.
The pattern of TGF beta expression and in vitro response to TGF beta has been defined in three ovarian carcinoma cell lines (PEO1, PEO4 and PEO14). Marked differences in both mRNA expression and growth responses were detected between the cell lines. All expressed mRNA for TGF beta 3, PEO1 and PEO4 but not PEO14 expressed mRNA for TGF beta 1, whereas PEO14 but not PEO1 and PEO4 expressed TGF beta 2. Growth of PEO14 cells in culture was markedly inhibited by both TGF beta 1 and beta 2. PEO1 cells were inhibited by TGF beta 1, but not TGF beta 2 whilst growth of PEO4 cells were not affected by exposure to either of these peptides. These data indicate that several elements of potential autocrine loops involving TGF beta's are present within ovarian cancer cells.
在三种卵巢癌细胞系（PEO1、PEO4和PEO14）中已确定了转化生长因子β（TGFβ）的表达模式以及对TGFβ的体外反应。在这些细胞系之间检测到mRNA表达和生长反应存在明显差异。所有细胞系均表达TGFβ3的mRNA，PEO1和PEO4表达TGFβ1的mRNA，但PEO14不表达，而PEO14表达TGFβ2的mRNA，PEO1和PEO4不表达。TGFβ1和β2均显著抑制PEO14细胞在培养中的生长。TGFβ1可抑制PEO1细胞，但TGFβ2不能，而PEO4细胞的生长不受这两种肽的影响。这些数据表明，卵巢癌细胞内存在涉及TGFβ的潜在自分泌环的多个要素。
