Derynck R, Jarrett J A, Chen E Y, Eaton D H, Bell J R, Assoian R K, Roberts A B, Sporn M B, Goeddel D V
Nature. 1985;316(6030):701-5. doi: 10.1038/316701a0.
The partial amino-acid sequence of purified human transforming growth factor-beta (TGF-beta) was used to identify a series of cDNA clones encoding the protein. The cDNA sequence indicates that the 112-amino acid monomeric form of the natural TGF-beta homodimer is derived proteolytically from a much longer precursor polypeptide which may be secreted. TGF-beta messenger RNA is synthesized in various normal and transformed cells.
利用纯化的人转化生长因子β（TGF-β）的部分氨基酸序列来鉴定一系列编码该蛋白的cDNA克隆。cDNA序列表明，天然TGF-β同二聚体的112个氨基酸单体形式是通过蛋白水解作用从一个可能分泌的长得多的前体多肽衍生而来。TGF-β信使核糖核酸在各种正常细胞和转化细胞中合成。
