Zhang Bao-Wei, Li Ming, Ma Li-Chao, Wei Fu-Wen
Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuan Xilu, Haidian, Beijing, PR China.
Biochem Genet. 2006 Dec;44(11-12):503-12. doi: 10.1007/s10528-006-9050-1.
Feces are increasingly used as sources of DNA for genetic and ecological research. This paper describes a new method for isolation of DNA from animal feces. This method combines multiple purification steps, including pretreatment with ethanol and TE, an inhibitor-absorber made of starch, the CTAB method, the phenol-chloroform extraction method, and the guanidinium thiocyanate-silica method. The new method is efficient according to PCR results of 585 fecal samples from 23 species and costs much less than the commercial kits. The protocol can be tailored to the specific purpose of examining different diets of animals and can be performed with routine laboratory reagents.
粪便越来越多地被用作遗传和生态研究的DNA来源。本文描述了一种从动物粪便中分离DNA的新方法。该方法结合了多个纯化步骤，包括用乙醇和TE进行预处理、使用由淀粉制成的抑制剂吸收剂、CTAB法、酚-氯仿萃取法和硫氰酸胍-硅胶法。根据来自23个物种的585份粪便样本的PCR结果，该新方法效率高，且成本远低于商业试剂盒。该方案可根据检查动物不同饮食的特定目的进行调整，并且可以使用常规实验室试剂进行操作。
