Arsura M, Introna M, Passerini F, Mantovani A, Golay J
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Blood. 1992 May 15;79(10):2708-16.
The B-myb gene is highly homologous to the c-myb protooncogene in several domains and also shares some of the functions of c-myb in that it can act as a transcriptional activator. In addition, the expression of both the B-myb and c-myb genes correlates with proliferation of normal hematopoietic cells. We investigated more directly the role of B-myb in proliferation of hematopoietic cell lines using B-myb-specific antisense oligonucleotides. We showed that several anti-B-myb oligonucleotides, complementary to distinct regions of the gene, inhibit significantly and in a dose-dependent manner the proliferation of all myeloid or lymphoid cell lines tested. This block in proliferation was not accompanied by detectable differentiation of U937 or HL60 cells to macrophages or granulocytes either spontaneously or after exposure to chemical agents. These data suggest that the B-myb gene, like c-myb, is necessary for hematopoietic cell proliferation.
B-myb基因在几个结构域中与c-myb原癌基因高度同源，并且还具有一些c-myb的功能，因为它可以作为转录激活因子。此外，B-myb和c-myb基因的表达都与正常造血细胞的增殖相关。我们使用B-myb特异性反义寡核苷酸更直接地研究了B-myb在造血细胞系增殖中的作用。我们发现，几种与该基因不同区域互补的抗B-myb寡核苷酸，以剂量依赖的方式显著抑制了所有测试的髓系或淋巴系细胞系的增殖。U937或HL60细胞无论是自发地还是在暴露于化学试剂后，向巨噬细胞或粒细胞的分化均未伴随这种增殖阻滞。这些数据表明，B-myb基因与c-myb一样，对造血细胞增殖是必需的。
