Brown Randall H, Gross Samuel S, Brent Michael R
Laboratory for Computational Genomics, Washington University, St. Louis, MO 63130, USA.
Genome Res. 2005 May;15(5):742-7. doi: 10.1101/gr.3696205.
The retrainable, comparative gene predictor N-SCAN integrates multigenome modeling and 5' untranslated region (5' UTR) modeling. In this article, we evaluate N-SCAN's transcription-start site (TSS) and first exon predictions both computationally and experimentally. The computational results indicate that N-SCAN is more accurate than any of the other tools we tested at predicting the TSS and the complete first exon. It is the only one of these tools that can predict complete gene structures together with 5' UTRs. Experimental evaluation shows that N-SCAN can be used to validate novel UTR introns in human gene predictions that do not overlap any RefSeq gene and even to correct RefSeq mRNAs by adding validated UTR exons that are missing from RefSeq.
可重新训练的比较基因预测器N-SCAN整合了多基因组建模和5'非翻译区（5'UTR）建模。在本文中，我们通过计算和实验评估了N-SCAN对转录起始位点（TSS）和首个外显子的预测。计算结果表明，在预测TSS和完整的首个外显子时，N-SCAN比我们测试的任何其他工具都更准确。它是这些工具中唯一能够预测完整基因结构以及5'UTR的工具。实验评估表明，N-SCAN可用于验证人类基因预测中不与任何RefSeq基因重叠的新型UTR内含子，甚至通过添加RefSeq中缺失的经过验证的UTR外显子来校正RefSeq mRNA。
