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用于检测在委内瑞拉苏克雷州皮肤利什曼病流行地区捕获的罗蛉属中自然利什曼原虫感染的多重聚合酶链反应。

Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela.

作者信息

Jorquera Alicia, González Ricardo, Marchán-Marcano Edgar, Oviedo Milagros, Matos Mercedes

机构信息

Centro de Investigaciones en Ciencias de la Salud, Universidad de Oriente, Núcleo de Anzoátegui, Barcelona, Venezuela.

出版信息

Mem Inst Oswaldo Cruz. 2005 Feb;100(1):45-8. doi: 10.1590/s0074-02762005000100008. Epub 2005 Apr 12.

DOI:10.1590/s0074-02762005000100008
PMID:15867962
Abstract

We studied the natural infection of Lutzomyia (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sand flies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captured, only two species of phlebotomines were identified: L. ovallesi (82.75%) and L. gomezi (17.42%). A sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-PCR assay for simultaneous detection of New Word Leishmaniaand Viannia subgenera. The results showed a total of 8 pools (15.68%) infected; of these, 7 were L. ovallesi naturally infected with L. braziliensis (2 pools) and L. mexicana (5 pools) and 1 pool of L. gomezi infected by L. braziliensis.

摘要

我们研究了委内瑞拉苏克雷州帕里亚半岛皮肤利什曼病流行区中华按蚊（Lutzomyia）属物种感染利什曼原虫的自然情况。2001年3月至2003年6月期间，使用香农诱虫灯和人饵收集了白蛉。在捕获的1291只昆虫中，仅鉴定出两种白蛉：卵形按蚊（L. ovallesi，占82.75%）和戈氏按蚊（L. gomezi，占17.42%）。通过多重聚合酶链反应（multiplex-PCR）分析收集的白蛉样本（51组，每组2至12只个体），以同时检测新大陆利什曼原虫和维氏亚属。结果显示，共有8组（占15.68%）被感染；其中，7组为卵形按蚊自然感染巴西利什曼原虫（2组）和墨西哥利什曼原虫（5组），1组戈氏按蚊感染巴西利什曼原虫。

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