Woodfine Kathryn, Carter Nigel P, Dunham Ian, Fiegler Heike
Department of Medical and Molecular Genetics, GKT School of Medicine, King's College London, London, SE1 9RT, UK.
Chromosome Res. 2005;13(3):249-57. doi: 10.1007/s10577-005-1504-5.
DNA microarrays are increasingly being used to investigate the functional role of chromatin. These studies are enhanced by the development of high-resolution arrays covering either the whole genome or specific regions of selected chromosomes with large insert clones, PCR products or oligonucleotides of around 100 bp or less. In combination with chromatin immunoprecipitation, this approach allows identification of protein binding for transcription factors, proteins involved in DNA replication and repair as well as sites of chromatin modification. Furthermore, by application of S phase fractions to genomic microarrays, replication timing can be estimated. Thus, microarrays can provide new information about chromosome structure and gene regulation.
DNA微阵列正越来越多地用于研究染色质的功能作用。覆盖整个基因组或选定染色体特定区域的高分辨率阵列的发展，通过使用大型插入克隆、PCR产物或约100bp或更短的寡核苷酸得以加强。结合染色质免疫沉淀，这种方法能够识别转录因子的蛋白质结合、参与DNA复制和修复的蛋白质以及染色质修饰位点。此外，通过将S期组分应用于基因组微阵列，可以估计复制时间。因此，微阵列可以提供有关染色体结构和基因调控的新信息。
