利用果蝇基因组平铺路径微阵列进行蛋白质 - DNA 相互作用图谱分析
Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.
作者信息
Sun Ling V, Chen Liang, Greil Frauke, Negre Nicolas, Li Tong-Ruei, Cavalli Giacomo, Zhao Hongyu, Van Steensel Bas, White Kevin P
机构信息
Department of Genetics and Biostatistics Division, Yale University School of Medicine, New Haven, CT 06520, USA.
出版信息
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9428-33. doi: 10.1073/pnas.1533393100. Epub 2003 Jul 22.
Abstract
We demonstrate the use of a chromosomal walk (or "tiling path") printed as DNA microarrays for mapping protein-DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430-920 bp in length, covering 2.9 million base pairs of the Adh-cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein-DNA interactions throughout these regions of the Drosophila genome.
摘要
我们展示了一种作为DNA微阵列打印的染色体步移（或“平铺路径”）的用途，用于绘制黑腹果蝇连续基因组DNA大片段上的蛋白质-DNA相互作用图谱。构建了包含长度为430 - 920 bp的基因组DNA片段的微阵列，覆盖了2号染色体Adh - cactus区域的290万个碱基对和3号染色体82F区域的85000个碱基对。我们对异染色质蛋白HP1和序列特异性GAGA转录因子进行了DNA定位图谱绘制，生成了果蝇基因组这些区域内体内蛋白质-DNA相互作用的全面、高分辨率图谱。
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