• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.利用果蝇基因组平铺路径微阵列进行蛋白质 - DNA 相互作用图谱分析
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9428-33. doi: 10.1073/pnas.1533393100. Epub 2003 Jul 22.
2
Chromatin profiling using targeted DNA adenine methyltransferase.使用靶向DNA腺嘌呤甲基转移酶的染色质分析
Nat Genet. 2001 Mar;27(3):304-8. doi: 10.1038/85871.
3
Mapping Drosophila genomic aberration breakpoints with comparative genome hybridization on microarrays.
Methods Enzymol. 2006;410:377-86. doi: 10.1016/S0076-6879(06)10018-X.
4
Genome-wide mapping of in vivo protein-DNA interactions.体内蛋白质-DNA相互作用的全基因组图谱绘制。
Science. 2007 Jun 8;316(5830):1497-502. doi: 10.1126/science.1141319. Epub 2007 May 31.
5
Genome-wide HP1 binding in Drosophila: developmental plasticity and genomic targeting signals.果蝇全基因组范围内的HP1结合：发育可塑性与基因组靶向信号
Genome Res. 2005 Sep;15(9):1265-73. doi: 10.1101/gr.3198905. Epub 2005 Aug 18.
6
DamID: mapping of in vivo protein-genome interactions using tethered DNA adenine methyltransferase.DamID：利用拴系DNA腺嘌呤甲基转移酶绘制体内蛋白质-基因组相互作用图谱
Methods Enzymol. 2006;410:342-59. doi: 10.1016/S0076-6879(06)10016-6.
7
Analysis of chromosome breakpoints in neuroblastoma at sub-kilobase resolution using fine-tiling oligonucleotide array CGH.使用精细平铺寡核苷酸阵列比较基因组杂交技术在亚千碱基分辨率下分析神经母细胞瘤中的染色体断点。
Genes Chromosomes Cancer. 2005 Nov;44(3):305-19. doi: 10.1002/gcc.20243.
8
Investigating chromosome organization with genomic microarrays.利用基因组微阵列研究染色体组织。
Chromosome Res. 2005;13(3):249-57. doi: 10.1007/s10577-005-1504-5.
9
Binding sites for metabolic disease related transcription factors inferred at base pair resolution by chromatin immunoprecipitation and genomic microarrays.通过染色质免疫沉淀和基因组微阵列以碱基对分辨率推断出的代谢疾病相关转录因子的结合位点。
Hum Mol Genet. 2005 Nov 15;14(22):3435-47. doi: 10.1093/hmg/ddi378. Epub 2005 Oct 12.
10
Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment.通过基因组富集的序列标签分析绘制大型基因组中的DNA-蛋白质相互作用图谱。
Nat Methods. 2005 Jan;2(1):47-53. doi: 10.1038/nmeth726. Epub 2004 Dec 21.

引用本文的文献

1
The Alk receptor tyrosine kinase regulates Sparkly, a novel activity regulating neuropeptide precursor in the central nervous system.Alk 受体酪氨酸激酶调控 Sparkly，一种中枢神经系统中新的活性调节神经肽前体。
Elife. 2024 Jun 21;12:RP88985. doi: 10.7554/eLife.88985.
2
DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm.DamID 转录谱分析鉴定出 Snail/Scratch 转录因子 Kahuli 是果蝇内脏中胚层中 Alk 的靶标。
Development. 2021 Dec 1;148(23). doi: 10.1242/dev.199465. Epub 2021 Dec 14.
3
Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner.深入细节：以细胞类型特异性方式分析基因表达和蛋白质-DNA相互作用
Adv Genet. 2015;91:103-151. doi: 10.1016/bs.adgen.2015.06.003. Epub 2015 Jul 23.
4
Hematopoietic transcriptional mechanisms: from locus-specific to genome-wide vantage points.造血转录机制：从特定基因座到全基因组的视角。
Exp Hematol. 2014 Aug;42(8):618-29. doi: 10.1016/j.exphem.2014.05.004. Epub 2014 May 9.
5
Cell-type-specific profiling of gene expression and chromatin binding without cell isolation: assaying RNA Pol II occupancy in neural stem cells.无需细胞分离即可进行细胞类型特异性的基因表达和染色质结合分析：检测神经干细胞中 RNA 聚合酶 II 的占有率。
Dev Cell. 2013 Jul 15;26(1):101-12. doi: 10.1016/j.devcel.2013.05.020. Epub 2013 Jun 20.
6
GeSICA: genome segmentation from intra-chromosomal associations.GeSICA：基于染色体内关联的基因组分割。
BMC Genomics. 2012 May 4;13:164. doi: 10.1186/1471-2164-13-164.
7
De-novo discovery of differentially abundant transcription factor binding sites including their positional preference.从头发现差异丰度转录因子结合位点，包括其位置偏好。
PLoS Comput Biol. 2011 Feb 10;7(2):e1001070. doi: 10.1371/journal.pcbi.1001070.
8
On the power and limits of evolutionary conservation--unraveling bacterial gene regulatory networks.论进化保守性的作用和局限——解析细菌基因调控网络。
Nucleic Acids Res. 2010 Dec;38(22):7877-84. doi: 10.1093/nar/gkq699. Epub 2010 Aug 10.
9
Neural stem cell transcriptional networks highlight genes essential for nervous system development.神经干细胞转录网络突出了神经系统发育所必需的基因。
EMBO J. 2009 Dec 16;28(24):3799-807. doi: 10.1038/emboj.2009.309.
10
Double error shrinkage method for identifying protein binding sites observed by tiling arrays with limited replication.双误差收缩法鉴定有限重复的平铺阵列观察到的蛋白质结合位点。
Bioinformatics. 2009 Oct 1;25(19):2486-91. doi: 10.1093/bioinformatics/btp471. Epub 2009 Aug 10.

本文引用的文献

1
Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.黑腹果蝇常染色体基因组注释：一项系统综述。
Genome Biol. 2002;3(12):RESEARCH0083. doi: 10.1186/gb-2002-3-12-research0083. Epub 2002 Dec 31.
2
Transcriptional regulatory networks in Saccharomyces cerevisiae.酿酒酵母中的转录调控网络。
Science. 2002 Oct 25;298(5594):799-804. doi: 10.1126/science.1075090.
3
Gene expression during the life cycle of Drosophila melanogaster.黑腹果蝇生命周期中的基因表达。
Science. 2002 Sep 27;297(5590):2270-5. doi: 10.1126/science.1072152.
4
Identification of regulatory elements using a feature selection method.使用特征选择方法识别调控元件。
Bioinformatics. 2002 Sep;18(9):1167-75. doi: 10.1093/bioinformatics/18.9.1167.
5
Developmental genomic approaches in model organisms.
Annu Rev Genomics Hum Genet. 2002;3:153-78. doi: 10.1146/annurev.genom.3.031302.100922. Epub 2002 Apr 15.
6
An algorithm for finding protein-DNA binding sites with applications to chromatin-immunoprecipitation microarray experiments.一种用于寻找蛋白质-DNA结合位点的算法及其在染色质免疫沉淀微阵列实验中的应用。
Nat Biotechnol. 2002 Aug;20(8):835-9. doi: 10.1038/nbt717. Epub 2002 Jul 8.
7
GATA-1 binding sites mapped in the beta-globin locus by using mammalian chIp-chip analysis.通过哺乳动物染色质免疫沉淀芯片分析绘制出β-珠蛋白基因座中的GATA-1结合位点。
Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2924-9. doi: 10.1073/pnas.052706999. Epub 2002 Feb 26.
8
E2F integrates cell cycle progression with DNA repair, replication, and G(2)/M checkpoints.E2F将细胞周期进程与DNA修复、复制及G(2)/M 检查点整合在一起。
Genes Dev. 2002 Jan 15;16(2):245-56. doi: 10.1101/gad.949802.
9
Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis.通过结合染色质免疫沉淀和CpG岛微阵列分析来分离人类转录因子靶点。
Genes Dev. 2002 Jan 15;16(2):235-44. doi: 10.1101/gad.943102.
10
Chromatin profiling using targeted DNA adenine methyltransferase.使用靶向DNA腺嘌呤甲基转移酶的染色质分析
Nat Genet. 2001 Mar;27(3):304-8. doi: 10.1038/85871.

利用果蝇基因组平铺路径微阵列进行蛋白质 - DNA 相互作用图谱分析

Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.

作者信息

Sun Ling V, Chen Liang, Greil Frauke, Negre Nicolas, Li Tong-Ruei, Cavalli Giacomo, Zhao Hongyu, Van Steensel Bas, White Kevin P

机构信息

Department of Genetics and Biostatistics Division, Yale University School of Medicine, New Haven, CT 06520, USA.

出版信息

Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9428-33. doi: 10.1073/pnas.1533393100. Epub 2003 Jul 22.

DOI:10.1073/pnas.1533393100
PMID:12876199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170935/
Abstract

We demonstrate the use of a chromosomal walk (or "tiling path") printed as DNA microarrays for mapping protein-DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430-920 bp in length, covering 2.9 million base pairs of the Adh-cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein-DNA interactions throughout these regions of the Drosophila genome.

摘要

我们展示了一种作为DNA微阵列打印的染色体步移（或“平铺路径”）的用途，用于绘制黑腹果蝇连续基因组DNA大片段上的蛋白质-DNA相互作用图谱。构建了包含长度为430 - 920 bp的基因组DNA片段的微阵列，覆盖了2号染色体Adh - cactus区域的290万个碱基对和3号染色体82F区域的85000个碱基对。我们对异染色质蛋白HP1和序列特异性GAGA转录因子进行了DNA定位图谱绘制，生成了果蝇基因组这些区域内体内蛋白质-DNA相互作用的全面、高分辨率图谱。